Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 248750..249252 Replicon chromosome
Accession NZ_CP087290
Organism Escherichia coli strain APEC 102026

Toxin (Protein)


Gene name yoeB Uniprot ID D3GZ85
Locus tag LO741_RS01245 Protein ID WP_000767820.1
Coordinates 248750..249004 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag LO741_RS01250 Protein ID WP_001259255.1
Coordinates 249001..249252 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO741_RS01215 (243765) 243765..243992 - 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
LO741_RS01220 (244006) 244006..245064 - 1059 WP_000492324.1 thiosulfate utilization transporter TsuA/YeeE -
LO741_RS01225 (245243) 245243..246601 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
LO741_RS01230 (246591) 246591..246653 - 63 WP_010723108.1 membrane protein YoeI -
LO741_RS01235 (246868) 246868..247797 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
LO741_RS01240 (247843) 247843..248667 - 825 WP_000754756.1 SDR family oxidoreductase -
LO741_RS01245 (248750) 248750..249004 - 255 WP_000767820.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
LO741_RS01250 (249001) 249001..249252 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
LO741_RS01255 (249536) 249536..249586 + 51 WP_001364200.1 his operon leader peptide -
LO741_RS01260 (249732) 249732..250631 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
LO741_RS01265 (250637) 250637..251941 + 1305 WP_039025710.1 histidinol dehydrogenase -
LO741_RS01270 (251938) 251938..253008 + 1071 WP_000108952.1 histidinol-phosphate transaminase -
LO741_RS01275 (253008) 253008..254075 + 1068 WP_000080077.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10182.64 Da        Isoelectric Point: 8.0353

>T225251 WP_000767820.1 NZ_CP087290:c249004-248750 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKIIELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T225251 NZ_CP121087:1809487-1809705 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT225251 WP_001259255.1 NZ_CP087290:c249252-249001 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT225251 NZ_CP121087:1809087-1809461 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D3GZ85

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References