Detailed information of TA system TA225244

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 148599..149024
• Replicon: plasmid p16_1068_1
• Accession: NZ_CP087288
• Organism: Escherichia coli strain APEC 16-1068

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LO742_RS25905
• Protein ID: WP_223195199.1
• Coordinates: 148599..148721 (-)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	148813..149024 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO742_RS25870 (143949)	143949..144176	-	228	WP_001442099.1	conjugal transfer relaxosome protein TraY	-
LO742_RS25875 (144270)	144270..144956	-	687	WP_000332490.1	PAS domain-containing protein	-
LO742_RS25880 (145146)	145146..145529	-	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LO742_RS25885 (145851)	145851..146453	+	603	WP_023145258.1	transglycosylase SLT domain-containing protein	-
LO742_RS25890 (146749)	146749..147570	-	822	WP_001234485.1	DUF932 domain-containing protein	-
LO742_RS25895 (147689)	147689..147975	-	287	Protein_153	hypothetical protein	-
LO742_RS25900 (147977)	147977..148298	+	322	Protein_154	hypothetical protein	-
LO742_RS25905 (148599)	148599..148721	-	123	WP_223195199.1	Hok/Gef family protein	Toxin
LO742_RS25910 (148666)	148666..148779	-	114	Protein_156	DUF5431 family protein	-
- (148813)	148813..149024	-	212	NuclAT_0	-	Antitoxin
- (148813)	148813..149024	-	212	NuclAT_0	-	Antitoxin
- (148813)	148813..149024	-	212	NuclAT_0	-	Antitoxin
- (148813)	148813..149024	-	212	NuclAT_0	-	Antitoxin
LO742_RS25915 (149035)	149035..149754	-	720	WP_001276275.1	plasmid SOS inhibition protein A	-
LO742_RS25920 (149751)	149751..150185	-	435	WP_000845935.1	conjugation system SOS inhibitor PsiB	-
LO742_RS25925 (150240)	150240..152198	-	1959	Protein_159	ParB/RepB/Spo0J family partition protein	-
LO742_RS25930 (152257)	152257..152490	-	234	WP_001209608.1	DUF905 domain-containing protein	-
LO742_RS25935 (152553)	152553..153050	-	498	WP_050427786.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..195721	195721
	-	flank	IS/Tn	-	-	153896..154399	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4536.35 Da
• Isoelectric Point: 8.2691

>T225244 WP_223195199.1 NZ_CP087288:c148721-148599 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTASLAYESSGK

Nucleotide

• Length: 123 bp

>T225244 NZ_CP121087:273620-273723 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 212 bp

>AT225244 NZ_CP087288:c149024-148813 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
AGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGC
AGAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGCCCCCTGTATGTCT

Structures

Antitoxin