Detailed information of TA system TA225241

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 110801..111055
• Replicon: plasmid p16_1068_1
• Accession: NZ_CP087288
• Organism: Escherichia coli strain APEC 16-1068

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LO742_RS25680
• Protein ID: WP_001312851.1
• Coordinates: 110801..110950 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	110994..111055 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO742_RS25645 (105885)	105885..106445	-	561	WP_001161490.1	recombinase family protein	-
LO742_RS25650 (106755)	106755..108332	+	1578	WP_001323889.1	PAS domain-containing methyl-accepting chemotaxis protein	-
LO742_RS25655 (108629)	108629..109486	-	858	WP_019842754.1	incFII family plasmid replication initiator RepA	-
LO742_RS25660 (109479)	109479..109961	-	483	WP_001273588.1	hypothetical protein	-
LO742_RS25665 (109954)	109954..110001	-	48	WP_229471593.1	hypothetical protein	-
LO742_RS25670 (109992)	109992..110243	+	252	WP_223195197.1	replication protein RepA	-
LO742_RS25675 (110260)	110260..110517	-	258	WP_000083845.1	replication regulatory protein RepA	-
LO742_RS25680 (110801)	110801..110950	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (110994)	110994..111055	+	62	NuclAT_1	-	Antitoxin
- (110994)	110994..111055	+	62	NuclAT_1	-	Antitoxin
- (110994)	110994..111055	+	62	NuclAT_1	-	Antitoxin
- (110994)	110994..111055	+	62	NuclAT_1	-	Antitoxin
LO742_RS25685 (111311)	111311..111385	-	75	Protein_111	endonuclease	-
LO742_RS25690 (111631)	111631..111843	-	213	WP_001299730.1	ANR family transcriptional regulator	-
LO742_RS25695 (111979)	111979..112539	-	561	WP_000139315.1	fertility inhibition protein FinO	-
LO742_RS25700 (112642)	112642..113502	-	861	WP_000704513.1	alpha/beta hydrolase	-
LO742_RS25705 (113561)	113561..114307	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..195721	195721
	-	inside	IScluster/Tn	-	-	97330..106445	9115

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T225241 WP_001312851.1 NZ_CP087288:c110950-110801 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T225241 NZ_CP121077:28886-29368 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGAAATAAGTGTTACTGCACCGGAGTTGCTAAACGAGGAACACTATCTTCAACAGTTTGATTGCGGGAATGATGTGCT
CAGTGACTGGCTTCGCCGCAGAGCGATGAAGAACCAATACCTCAATGCCTCCCGTACATTTGTTATCTGCCCTGAAGGCA
CAAAGCGAGTAGTGGGATATTACTCTATTGCGACGGGGTCTGTAAGTCATGCCAGTTTAGGTCGCAGTTTACGCCAGAAT
ATGCCCGATCCTGTTCCGGTTGTTTTGCTGGGACGTCTTGCTGTTGATGAGTGTACTCAAGGGCATAGTTTTGGAAAATG
GCTGCTTAATGATGCTGTAACACGGGTATCTAACCTGGCAGACCAGGTGGGTATTAAAGCGATCATGGTTCATGCTATTG
ATGAACAGGCGAAAACATTCTATGAATACTTTGGCTTTGTTCAGTCTCCCATTGCGCCAAATACATTGTTCTACAAGATT
TAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT225241 NZ_CP087288:110994-111055 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin