Detailed information of TA system TA225239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5051425..5052027
• Replicon: chromosome
• Accession: NZ_CP087287
• Organism: Escherichia coli strain APEC 16-1068

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: LO742_RS24970
• Protein ID: WP_000897302.1
• Coordinates: 5051425..5051736 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LO742_RS24975
• Protein ID: WP_000356397.1
• Coordinates: 5051737..5052027 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO742_RS24945 (5047339)	5047339..5047938	+	600	WP_001314324.1	glucose-1-phosphatase	-
LO742_RS24950 (5047932)	5047932..5048804	+	873	WP_000920754.1	virulence factor BrkB family protein	-
LO742_RS24955 (5048801)	5048801..5049238	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
LO742_RS24960 (5049283)	5049283..5050224	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
LO742_RS24965 (5050288)	5050288..5051196	-	909	WP_061091392.1	alpha/beta hydrolase	-
LO742_RS24970 (5051425)	5051425..5051736	+	312	WP_000897302.1	hypothetical protein	Toxin
LO742_RS24975 (5051737)	5051737..5052027	+	291	WP_000356397.1	NadS family protein	Antitoxin
LO742_RS24980 (5052386)	5052386..5052664	+	279	WP_001296612.1	hypothetical protein	-
LO742_RS24985 (5053060)	5053060..5053278	+	219	WP_001314326.1	CopG family transcriptional regulator	-
LO742_RS24990 (5053494)	5053494..5054423	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
LO742_RS24995 (5054420)	5054420..5055055	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
LO742_RS25000 (5055052)	5055052..5055954	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T225239 WP_000897302.1 NZ_CP087287:5051425-5051736 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T225239 NZ_CP121076:4660962-4661273 [Salmonella enterica subsp. enterica serovar Infantis]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT225239 WP_000356397.1 NZ_CP087287:5051737-5052027 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT225239 NZ_CP121076:4661270-4661716 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: S1P416