Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2862653..2863155 Replicon chromosome
Accession NZ_CP087287
Organism Escherichia coli strain APEC 16-1068

Toxin (Protein)


Gene name yoeB Uniprot ID A0A0H2Z0A6
Locus tag LO742_RS14360 Protein ID WP_000767826.1
Coordinates 2862653..2862907 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag LO742_RS14365 Protein ID WP_001259255.1
Coordinates 2862904..2863155 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO742_RS14330 (2857668) 2857668..2857895 - 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
LO742_RS14335 (2857909) 2857909..2858967 - 1059 WP_000492334.1 thiosulfate utilization transporter TsuA/YeeE -
LO742_RS14340 (2859146) 2859146..2860504 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
LO742_RS14345 (2860494) 2860494..2860556 - 63 WP_010723108.1 membrane protein YoeI -
LO742_RS14350 (2860771) 2860771..2861700 - 930 WP_000803363.1 LysR substrate-binding domain-containing protein -
LO742_RS14355 (2861746) 2861746..2862570 - 825 WP_000754737.1 SDR family oxidoreductase -
LO742_RS14360 (2862653) 2862653..2862907 - 255 WP_000767826.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
LO742_RS14365 (2862904) 2862904..2863155 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
LO742_RS14370 (2863625) 2863625..2864524 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
LO742_RS14375 (2864530) 2864530..2865834 + 1305 WP_001350692.1 histidinol dehydrogenase -
LO742_RS14380 (2865831) 2865831..2866901 + 1071 WP_000108997.1 histidinol-phosphate transaminase -
LO742_RS14385 (2866901) 2866901..2867968 + 1068 WP_000080128.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10195.64 Da        Isoelectric Point: 8.0353

>T225235 WP_000767826.1 NZ_CP087287:c2862907-2862653 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRIIEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T225235 NZ_CP121076:3516622-3516840 [Salmonella enterica subsp. enterica serovar Infantis]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCTGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT225235 WP_001259255.1 NZ_CP087287:c2863155-2862904 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT225235 NZ_CP121076:3516220-3516594 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H2Z0A6

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References