Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 1212874..1213553 Replicon chromosome
Accession NZ_CP087287
Organism Escherichia coli strain APEC 16-1068

Toxin (Protein)


Gene name higB Uniprot ID V0VBP6
Locus tag LO742_RS05810 Protein ID WP_000057524.1
Coordinates 1212874..1213176 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag LO742_RS05815 Protein ID WP_000806442.1
Coordinates 1213212..1213553 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LO742_RS05785 (1208250) 1208250..1209902 + 1653 WP_000771762.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
LO742_RS05790 (1209940) 1209940..1210443 - 504 WP_000667000.1 hypothetical protein -
LO742_RS05795 (1210440) 1210440..1211240 - 801 WP_000439796.1 hypothetical protein -
LO742_RS05800 (1211264) 1211264..1211743 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
LO742_RS05805 (1211947) 1211947..1212741 - 795 WP_000365177.1 TraB/GumN family protein -
LO742_RS05810 (1212874) 1212874..1213176 + 303 WP_000057524.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LO742_RS05815 (1213212) 1213212..1213553 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
LO742_RS05820 (1213611) 1213611..1216115 - 2505 WP_000083945.1 copper-exporting P-type ATPase CopA -
LO742_RS05825 (1216377) 1216377..1217309 + 933 WP_000883052.1 glutaminase A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1209940..1222154 12214


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11839.47 Da        Isoelectric Point: 10.2237

>T225220 WP_000057524.1 NZ_CP087287:1212874-1213176 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T225220 NZ_CP121075:1176225-1176638 [Salmonella enterica subsp. enterica serovar Infantis]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTTCTGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT225220 WP_000806442.1 NZ_CP087287:1213212-1213553 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT225220 NZ_CP121075:1175978-1176244 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0VBP6

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References