Detailed information of TA system TA225220

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 1212874..1213553
• Replicon: chromosome
• Accession: NZ_CP087287
• Organism: Escherichia coli strain APEC 16-1068

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V0VBP6
• Locus tag: LO742_RS05810
• Protein ID: WP_000057524.1
• Coordinates: 1212874..1213176 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: S1QAY3
• Locus tag: LO742_RS05815
• Protein ID: WP_000806442.1
• Coordinates: 1213212..1213553 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO742_RS05785 (1208250)	1208250..1209902	+	1653	WP_000771762.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
LO742_RS05790 (1209940)	1209940..1210443	-	504	WP_000667000.1	hypothetical protein	-
LO742_RS05795 (1210440)	1210440..1211240	-	801	WP_000439796.1	hypothetical protein	-
LO742_RS05800 (1211264)	1211264..1211743	-	480	WP_000186633.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
LO742_RS05805 (1211947)	1211947..1212741	-	795	WP_000365177.1	TraB/GumN family protein	-
LO742_RS05810 (1212874)	1212874..1213176	+	303	WP_000057524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LO742_RS05815 (1213212)	1213212..1213553	+	342	WP_000806442.1	HigA family addiction module antitoxin	Antitoxin
LO742_RS05820 (1213611)	1213611..1216115	-	2505	WP_000083945.1	copper-exporting P-type ATPase CopA	-
LO742_RS05825 (1216377)	1216377..1217309	+	933	WP_000883052.1	glutaminase A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1209940..1222154	12214

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-100)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11839.47 Da
• Isoelectric Point: 10.2237

>T225220 WP_000057524.1 NZ_CP087287:1212874-1213176 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHWDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Nucleotide

• Length: 303 bp

>T225220 NZ_CP121075:1176225-1176638 [Salmonella enterica subsp. enterica serovar Infantis]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTTCTGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13171.10 Da
• Isoelectric Point: 5.7790

>AT225220 WP_000806442.1 NZ_CP087287:1213212-1213553 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Nucleotide

• Length: 342 bp

>AT225220 NZ_CP121075:1175978-1176244 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: V0VBP6

Antitoxin

• AlphaFold DB: S1QAY3