Detailed information of TA system TA225218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 971529..972223
• Replicon: chromosome
• Accession: NZ_CP087287
• Organism: Escherichia coli strain APEC 16-1068

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: LO742_RS04670
• Protein ID: WP_001263500.1
• Coordinates: 971825..972223 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: LO742_RS04665
• Protein ID: WP_000554758.1
• Coordinates: 971529..971822 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO742_RS04640 (967065)	967065..967562	+	498	WP_000006241.1	REP-associated tyrosine transposase RayT	-
LO742_RS04645 (967645)	967645..967803	-	159	WP_014639450.1	hypothetical protein	-
LO742_RS04650 (967882)	967882..969594	-	1713	Protein_902	flagellar biosynthesis protein FlhA	-
LO742_RS04655 (969566)	969566..970351	+	786	WP_000207544.1	putative lateral flagellar export/assembly protein LafU	-
LO742_RS04660 (970422)	970422..971477	+	1056	WP_001226168.1	DNA polymerase IV	-
LO742_RS04665 (971529)	971529..971822	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
LO742_RS04670 (971825)	971825..972223	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
LO742_RS04675 (972233)	972233..972685	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
LO742_RS04680 (972875)	972875..974014	+	1140	WP_000521577.1	RNA ligase RtcB family protein	-
LO742_RS04685 (974011)	974011..974625	+	615	WP_000602123.1	peptide chain release factor H	-
LO742_RS04690 (974682)	974682..976139	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
LO742_RS04695 (976400)	976400..976858	+	459	WP_001291988.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	952003..972685	20682

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T225218 WP_001263500.1 NZ_CP087287:971825-972223 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T225218 NZ_CP121075:535260-535745 [Salmonella enterica subsp. enterica serovar Infantis]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT225218 WP_000554758.1 NZ_CP087287:971529-971822 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT225218 NZ_CP121075:534985-535272 [Salmonella enterica subsp. enterica serovar Infantis]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCAGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3