Detailed information of TA system TA225210

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 71268..71995
• Replicon: plasmid pYUSHP29-2
• Accession: NZ_CP087282
• Organism: Leclercia adecarboxylata strain SH19PE29

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A482LZ08
• Locus tag: LO739_RS23780
• Protein ID: WP_045898923.1
• Coordinates: 71268..71579 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LO739_RS23785
• Protein ID: WP_102752378.1
• Coordinates: 71576..71995 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LO739_RS23765 (LO739_23760)	66740..67418	-	679	Protein_73	IS3 family transposase	-
LO739_RS23770 (LO739_23765)	67575..69407	+	1833	WP_172693811.1	methyl-accepting chemotaxis protein	-
LO739_RS23775 (LO739_23770)	69527..70647	+	1121	WP_172693812.1	IS3 family transposase	-
LO739_RS23780 (LO739_23775)	71268..71579	+	312	WP_045898923.1	type II toxin-antitoxin system HigB family toxin	Toxin
LO739_RS23785 (LO739_23780)	71576..71995	+	420	WP_102752378.1	helix-turn-helix domain-containing protein	Antitoxin
LO739_RS23790 (LO739_23785)	72158..73305	+	1148	WP_230431009.1	IS3 family transposase	-
LO739_RS23795 (LO739_23790)	73372..73683	+	312	WP_230431557.1	fimbrial protein	-
LO739_RS23800 (LO739_23795)	73830..74949	-	1120	Protein_80	IS3-like element ISKpn34 family transposase	-
LO739_RS23805 (LO739_23800)	75120..75245	-	126	WP_230431592.1	hypothetical protein	-
LO739_RS23810 (LO739_23805)	75583..76068	-	486	WP_172693814.1	GNAT family N-acetyltransferase	-
LO739_RS23815 (LO739_23810)	76056..76322	-	267	WP_172693815.1	DUF1778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..132089	132089
	-	inside	IScluster/Tn	-	-	62065..74949	12884

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12347.07 Da
• Isoelectric Point: 9.7248

>T225210 WP_045898923.1 NZ_CP087282:71268-71579 [Leclercia adecarboxylata]
MHVISKEPFEEAAKQYPNDSLAVRALYRLVRETDFSSPAEMRTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T225210 NZ_CP121073:c4660131-4659820 [Salmonella enterica subsp. enterica serovar Infantis]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15507.68 Da
• Isoelectric Point: 4.3573

>AT225210 WP_102752378.1 NZ_CP087282:71576-71995 [Leclercia adecarboxylata]
MITDTAKAIEATKQLIAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEMPV
GVALLRTLIDQYRLSYSDLKEEIGSKSLVSQILSGQRSLTIMHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT225210 NZ_CP121073:c4659819-4659529 [Salmonella enterica subsp. enterica serovar Infantis]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A482LZ08