Detailed information of TA system TA225194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 734259..734911
• Replicon: chromosome
• Accession: NZ_CP087278
• Organism: Ralstonia solanacearum strain FJ1003

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0S4U5H4
• Locus tag: LOH50_RS20700
• Protein ID: WP_028861315.1
• Coordinates: 734259..734603 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOH50_RS20705
• Protein ID: WP_020425351.1
• Coordinates: 734600..734911 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOH50_RS20685	729955..731118	-	1164	WP_016726526.1	2-methylcitrate synthase	-
LOH50_RS20690	731170..732066	-	897	WP_071092368.1	methylisocitrate lyase	-
LOH50_RS20695	732259..734208	+	1950	WP_023470006.1	propionate catabolism operon regulatory protein PrpR	-
LOH50_RS20700	734259..734603	+	345	WP_028861315.1	hypothetical protein	Toxin
LOH50_RS20705	734600..734911	+	312	WP_020425351.1	transcriptional regulator	Antitoxin
LOH50_RS20710	735051..735860	-	810	WP_071623829.1	DUF2242 domain-containing protein	-
LOH50_RS20715	736504..737079	-	576	WP_071623824.1	hypothetical protein	-
LOH50_RS20720	737492..739339	+	1848	WP_071623825.1	3'-5' exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	734259..754204	19945

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13276.03 Da
• Isoelectric Point: 9.3395

>T225194 WP_028861315.1 NZ_CP087278:734259-734603 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLARPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGGGQFWM
FVVYDKGEADDLSSDERKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T225194 NZ_CP121070:4661607-4661918 [Salmonella enterica subsp. enterica serovar Infantis]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11619.65 Da
• Isoelectric Point: 10.7132

>AT225194 WP_020425351.1 NZ_CP087278:734600-734911 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVKVRVLQPLDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT225194 NZ_CP121070:4661915-4662361 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: A0A0S4U5H4