Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/COG4683-HTH_37 |
| Location | 83564..84221 | Replicon | plasmid plas2 |
| Accession | NZ_CP087274 | ||
| Organism | Klebsiella pneumoniae strain ATCC BAA 2146 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | U3PDC3 |
| Locus tag | LN739_RS28035 | Protein ID | WP_000270043.1 |
| Coordinates | 83564..83914 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | LN739_RS28040 | Protein ID | WP_000124640.1 |
| Coordinates | 83919..84221 (+) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LN739_RS28000 (LN739_28000) | 80038..80535 | - | 498 | WP_000062185.1 | hypothetical protein | - |
| LN739_RS28005 (LN739_28005) | 80538..81026 | - | 489 | WP_001273096.1 | DUF1643 domain-containing protein | - |
| LN739_RS28010 (LN739_28010) | 81123..81458 | + | 336 | WP_000683477.1 | hypothetical protein | - |
| LN739_RS28015 (LN739_28015) | 81473..81943 | - | 471 | WP_001281821.1 | hypothetical protein | - |
| LN739_RS28020 (LN739_28020) | 81936..82307 | - | 372 | WP_000516918.1 | hypothetical protein | - |
| LN739_RS28025 (LN739_28025) | 82318..82512 | - | 195 | WP_000343597.1 | hypothetical protein | - |
| LN739_RS28030 (LN739_28030) | 82853..83401 | - | 549 | WP_001061573.1 | hypothetical protein | - |
| LN739_RS28035 (LN739_28035) | 83564..83914 | + | 351 | WP_000270043.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| LN739_RS28040 (LN739_28040) | 83919..84221 | + | 303 | WP_000124640.1 | XRE family transcriptional regulator | Antitoxin |
| LN739_RS28045 (LN739_28045) | 84248..84541 | - | 294 | WP_001239998.1 | hypothetical protein | - |
| LN739_RS28050 (LN739_28050) | 84630..84902 | - | 273 | WP_001043046.1 | HU family DNA-binding protein | - |
| LN739_RS28055 (LN739_28055) | 84960..85487 | - | 528 | WP_004201083.1 | thermonuclease family protein | - |
| LN739_RS28060 (LN739_28060) | 85490..85681 | - | 192 | WP_001270409.1 | hypothetical protein | - |
| LN739_RS28065 (LN739_28065) | 85707..86564 | - | 858 | WP_001167036.1 | hypothetical protein | - |
| LN739_RS28070 (LN739_28070) | 86551..86781 | - | 231 | WP_000972665.1 | hypothetical protein | - |
| LN739_RS28075 (LN739_28075) | 86781..87299 | - | 519 | WP_000210757.1 | nitrite reductase | - |
| LN739_RS28080 (LN739_28080) | 87296..87742 | - | 447 | WP_000919343.1 | hypothetical protein | - |
| LN739_RS28085 (LN739_28085) | 87742..88101 | - | 360 | WP_000422769.1 | hypothetical protein | - |
| LN739_RS28090 (LN739_28090) | 88159..88587 | - | 429 | WP_000591076.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaCMY-6 / blaNDM-1 / rmtC / sul1 / qacE / aac(6')-Ib | htpB | 1..140824 | 140824 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 13328.21 Da Isoelectric Point: 5.2421
>T225189 WP_000270043.1 NZ_CP087274:83564-83914 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE
Download Length: 351 bp
>T225189 NZ_CP121070:c2643894-2643791 [Salmonella enterica subsp. enterica serovar Infantis]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT
Antitoxin
Download Length: 101 a.a. Molecular weight: 11006.73 Da Isoelectric Point: 7.2036
>AT225189 WP_000124640.1 NZ_CP087274:83919-84221 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV
Download Length: 303 bp
>AT225189 NZ_CP121070:2643789-2643932 [Salmonella enterica subsp. enterica serovar Infantis]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|