Detailed information of TA system TA225168

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 66287..66939
• Replicon: plasmid pHD6448-1
• Accession: NZ_CP087267
• Organism: Aeromonas veronii strain HD6448

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6I4WW70
• Locus tag: KXU59_RS24280
• Protein ID: WP_024943279.1
• Coordinates: 66287..66637 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KXU59_RS24285
• Protein ID: WP_024943280.1
• Coordinates: 66637..66939 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXU59_RS24255 (LOS88_24255)	61925..63012	+	1088	WP_088821966.1	IS3-like element ISAs22 family transposase	-
KXU59_RS24260 (LOS88_24260)	63043..63309	+	267	Protein_71	transposase	-
KXU59_RS24265 (LOS88_24265)	63587..64216	+	630	WP_024943276.1	hypothetical protein	-
KXU59_RS24270 (LOS88_24270)	64851..65840	+	990	WP_024943277.1	SEL1-like repeat protein	-
KXU59_RS24275 (LOS88_24275)	65983..66228	+	246	WP_024943278.1	hypothetical protein	-
KXU59_RS24280 (LOS88_24280)	66287..66637	+	351	WP_024943279.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KXU59_RS24285 (LOS88_24285)	66637..66939	+	303	WP_024943280.1	helix-turn-helix transcriptional regulator	Antitoxin
KXU59_RS24290 (LOS88_24290)	67207..68768	+	1562	WP_088813959.1	IS3-like element ISAs20 family transposase	-
KXU59_RS24295 (LOS88_24295)	68932..69564	+	633	WP_024943281.1	ParA family protein	-
KXU59_RS24300 (LOS88_24300)	69570..69863	+	294	WP_024943282.1	hypothetical protein	-
KXU59_RS24305 (LOS88_24305)	69986..70315	+	330	WP_162791158.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-Ib-cr / catB3 / blaCMY-8b / qacE / sul1	-	1..70555	70555
	-	inside	IScluster/Tn	aac(6')-Ib-cr / catB3 / blaCMY-8b / qacE / sul1	-	36173..68768	32595

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-81)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12937.79 Da
• Isoelectric Point: 5.7350

>T225168 WP_024943279.1 NZ_CP087267:66287-66637 [Aeromonas veronii]
MWTVKTTSLFDVWFDEQDEATQEKVLAGLLALAQGGPSVGRPLVDTIKGSRFTNLKELRVQHQGDPLRAFFAFDPLRQAI
VLCAGNKGGNDKRFYKEMLPVAEAEYAKHLTELEVG

Nucleotide

• Length: 351 bp

>T225168 NZ_CP121068:c1014221-1013694 [Salmonella enterica subsp. enterica serovar Infantis]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTACAACGCCATGCACTACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGCGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACAATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11137.84 Da
• Isoelectric Point: 5.1667

>AT225168 WP_024943280.1 NZ_CP087267:66637-66939 [Aeromonas veronii]
MGRTLNEMLASRSVDSQQRIQEMANELLLEVRLQALREELEVSQVELASALGISQPAVVAMEQRGNDIKLSTMKRYVEAM
GGKLRVDVELPTGRHVGFNV

Nucleotide

• Length: 303 bp

>AT225168 NZ_CP121068:c1014508-1014218 [Salmonella enterica subsp. enterica serovar Infantis]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGACTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: A0A6I4WW70