Detailed information of TA system TA225139

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 6259853..6260525
• Replicon: chromosome
• Accession: NZ_CP087204
• Organism: Pseudomonas sp. B21-054

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LOY70_RS27280
• Protein ID: WP_207284157.1
• Coordinates: 6260160..6260525 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: W8QJ57
• Locus tag: LOY70_RS27275
• Protein ID: WP_025215812.1
• Coordinates: 6259853..6260167 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY70_RS27260 (LOY70_27215)	6256067..6256747	+	681	WP_123416060.1	GntR family transcriptional regulator	-
LOY70_RS27265 (LOY70_27220)	6256957..6259320	+	2364	WP_265031561.1	hypothetical protein	-
LOY70_RS27270 (LOY70_27225)	6259356..6259817	+	462	WP_060743121.1	Lrp/AsnC family transcriptional regulator	-
LOY70_RS27275 (LOY70_27230)	6259853..6260167	-	315	WP_025215812.1	XRE family transcriptional regulator	Antitoxin
LOY70_RS27280 (LOY70_27235)	6260160..6260525	-	366	WP_207284157.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY70_RS27285 (LOY70_27240)	6260889..6262571	+	1683	WP_265031566.1	NAD(P)/FAD-dependent oxidoreductase	-
LOY70_RS27290 (LOY70_27245)	6262584..6263378	+	795	WP_265031567.1	carbon-nitrogen hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (5-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14181.91 Da
• Isoelectric Point: 5.2214

>T225139 WP_207284157.1 NZ_CP087204:c6260525-6260160 [Pseudomonas sp. B21-054]
MKWDVEYTDEFEMWWNRLCESEQDSVQASVMLLGDTGPYLGFPHTSDIKGSRHGNLRELRVQHAGRPYRVLYAFDPRRCA
ILLIGGDKTGQGRWYQEYVPLAERLYDEHLEVLKREGFDNG

Nucleotide

• Length: 366 bp

>T225139 NZ_CP121003:3411822-3412040 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11872.62 Da
• Isoelectric Point: 9.6213

>AT225139 WP_025215812.1 NZ_CP087204:c6260167-6259853 [Pseudomonas sp. B21-054]
MAKKFSDLMARRSPDSQARAQALYQTLRAEMPLHELRQAQELSQEALAKALHINQAAVSKMERRTDMYISTLRNYIRAMG
GELEIIATFPEGQVRIENFSHPTP

Nucleotide

• Length: 315 bp

>AT225139 NZ_CP121003:3411420-3411794 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1I5FD61