Detailed information of TA system TA225131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4289411..4290015
• Replicon: chromosome
• Accession: NZ_CP087203
• Organism: Pseudomonas sp. B21-056

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LOY67_RS18215
• Protein ID: WP_265063816.1
• Coordinates: 4289411..4289725 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOY67_RS18220
• Protein ID: WP_265063817.1
• Coordinates: 4289728..4290015 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY67_RS18195 (LOY67_18170)	4285820..4286062	-	243	WP_265063812.1	hypothetical protein	-
LOY67_RS18200 (LOY67_18175)	4286177..4287520	+	1344	WP_265063813.1	chromate efflux transporter	-
LOY67_RS18205 (LOY67_18180)	4287517..4287948	-	432	WP_265063814.1	arsenic resistance N-acetyltransferase ArsN2	-
LOY67_RS18210 (LOY67_18185)	4288143..4289297	+	1155	WP_265063815.1	mechanosensitive ion channel family protein	-
LOY67_RS18215 (LOY67_18190)	4289411..4289725	+	315	WP_265063816.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY67_RS18220 (LOY67_18195)	4289728..4290015	+	288	WP_265063817.1	helix-turn-helix domain-containing protein	Antitoxin
LOY67_RS18225 (LOY67_18200)	4290313..4290792	+	480	WP_265063818.1	hypothetical protein	-
LOY67_RS18230 (LOY67_18205)	4290803..4291051	-	249	WP_265063819.1	hypothetical protein	-
LOY67_RS18235 (LOY67_18210)	4291428..4292891	-	1464	WP_265063820.1	aspartate/glutamate racemase family protein	-
LOY67_RS18240 (LOY67_18215)	4292903..4294198	-	1296	WP_265063821.1	dicarboxylate/amino acid:cation symporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4289411..4297568	8157

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-87)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11846.63 Da
• Isoelectric Point: 9.8325

>T225131 WP_265063816.1 NZ_CP087203:4289411-4289725 [Pseudomonas sp. B21-056]
MIFIETPIFTRRANELMNDDDYSTLQATLVRNPSAGDVIEATGGIRKIRIAAKGHGKRGGARVIYYHFVADSQIALLMIY
PKNEQQDLTVDQRKAMKTIVEHWR

Nucleotide

• Length: 315 bp

>T225131 NZ_CP121003:200447-200932 [Salmonella enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGTGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10697.35 Da
• Isoelectric Point: 10.1758

>AT225131 WP_265063817.1 NZ_CP087203:4289728-4290015 [Pseudomonas sp. B21-056]
MSKFFDELMESVQQMDEIVRGERQPSRKFVVDALQVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRRAPTGPARALLR
AIHNDPEHVISALAV

Nucleotide

• Length: 288 bp

>AT225131 NZ_CP121003:200172-200459 [Salmonella enterica]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCAGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA