Detailed information of TA system TA225122

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2283031..2283624
• Replicon: chromosome
• Accession: NZ_CP087201
• Organism: Pseudomonas sp. B21-059

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LOY69_RS10485
• Protein ID: WP_265074465.1
• Coordinates: 2283346..2283624 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: LOY69_RS10480
• Protein ID: WP_265074464.1
• Coordinates: 2283031..2283336 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY69_RS10455 (LOY69_10440)	2278690..2278899	+	210	WP_265074459.1	hypothetical protein	-
LOY69_RS10460 (LOY69_10445)	2279016..2279660	+	645	WP_265074460.1	NAD-dependent DNA ligase	-
LOY69_RS10465 (LOY69_10450)	2280037..2281020	+	984	WP_265074461.1	P63C domain-containing protein	-
LOY69_RS10470 (LOY69_10455)	2281117..2281590	+	474	WP_265074462.1	hypothetical protein	-
LOY69_RS10475 (LOY69_10460)	2281647..2282876	-	1230	WP_265074463.1	hypothetical protein	-
LOY69_RS10480 (LOY69_10465)	2283031..2283336	-	306	WP_265074464.1	HigA family addiction module antitoxin	Antitoxin
LOY69_RS10485 (LOY69_10470)	2283346..2283624	-	279	WP_265074465.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY69_RS10490 (LOY69_10475)	2284434..2285291	+	858	WP_265074466.1	hypothetical protein	-
LOY69_RS10495 (LOY69_10480)	2285496..2285927	-	432	WP_265074467.1	helix-turn-helix domain-containing protein	-
LOY69_RS10500 (LOY69_10485)	2285957..2286238	+	282	WP_265074468.1	Arc family DNA-binding protein	-
LOY69_RS10505 (LOY69_10490)	2286312..2286620	+	309	WP_265074469.1	DUF3077 domain-containing protein	-
LOY69_RS10510 (LOY69_10495)	2286620..2287417	+	798	WP_265074470.1	phage antirepressor KilAC domain-containing protein	-
LOY69_RS10515 (LOY69_10500)	2287523..2288272	+	750	WP_265074471.1	zeta toxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2275622..2338361	62739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (6-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10256.73 Da
• Isoelectric Point: 9.1493

>T225122 WP_265074465.1 NZ_CP087201:c2283624-2283346 [Pseudomonas sp. B21-059]
MIISFGCGETESLYKTGSSRRWVGILSVAQRKLTMLDAATSIQDLKSPPGNRLEALQGNRKGQHSIRINDQFRICFIWGA
YGPESVAIVDYH

Nucleotide

• Length: 279 bp

>T225122 NZ_CP120978:c2470397-2469987 [Moellerella wisconsensis]
GTGGTCCTGTGGAAATCTAATTTATCCATATCATGGATGGCTCAGCTTTTTTCTACCTGTGTACATTGTGTCCTGGGGCT
AGTGTTATTACTGGCTCCGTGGCCGACAGGTAACTCTATTATTTGGCTTCCTTTATTAATGGTGACTATTGCGAGTTGGG
CAAAAAGTCAAAAAAACATTAATAAATACAAAGGAATAATTGTTCTTACTAATGGGAATAAAGTCCAATGGAAGAAAAAT
GAATGGAGTATTATCACTCCTCCATGGTGTACTCGCTTTGGTATTTTATTAACGTTAAGTGCATTACAAGGTAAACCTAG
AAAAATAAAATTATGGATTGCAGCTGATGCAATATCAGAAGACGATTGGCGAAATTTAAATCAGTTATTATTACAATATC
CTGATATATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11150.73 Da
• Isoelectric Point: 6.2108

>AT225122 WP_265074464.1 NZ_CP087201:c2283336-2283031 [Pseudomonas sp. B21-059]
VFINGMRPVHPGEILREEYLHPMDLSPAALAKLLNVSAPTVNDIVRGRRNISADVALRLAACFDTSPQFWANLQSAYDLR
KAEIDHGAEIAQQVQRAPECA

Nucleotide

• Length: 306 bp

>AT225122 NZ_CP120978:c2470644-2470378 [Moellerella wisconsensis]
ATGGATATTGATAATAAAGCGCGGATCCACTGGGCATGTCGTCGAGGAATGCGAGAACTAGATATTTCAATCATGCCTTT
TTTTGAACATGAGTATGATGGATTAAACGATAATGAAAAACACCAGTTTATTCGTTTGCTGGAGTGTGCAGATCCCGATC
TGTTCAATTGGTTGATGAACCATGGTCGTCCTGACGATGATGAGCTTTATCATATGATTAAACTAATTCAACAAAGAAAT
AAAGACCGTGGTCCTGTGGAAATCTAA