Detailed information of TA system TA225112

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5866910..5867582
• Replicon: chromosome
• Accession: NZ_CP087198
• Organism: Pseudomonas sp. B21-010

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LOY50_RS26245
• Protein ID: WP_214509864.1
• Coordinates: 5867217..5867582 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0F4XJJ2
• Locus tag: LOY50_RS26240
• Protein ID: WP_024616743.1
• Coordinates: 5866910..5867224 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY50_RS26215 (LOY50_26160)	5863140..5863382	+	243	WP_214509862.1	hypothetical protein	-
LOY50_RS26220 (LOY50_26165)	5863450..5863557	+	108	Protein_5159	DNA-binding protein	-
LOY50_RS26225 (LOY50_26170)	5863568..5863855	+	288	WP_253469952.1	hypothetical protein	-
LOY50_RS26230 (LOY50_26175)	5864014..5866368	+	2355	WP_258689543.1	DUF308 domain-containing protein	-
LOY50_RS26235 (LOY50_26180)	5866405..5866866	+	462	WP_018607342.1	Lrp/AsnC family transcriptional regulator	-
LOY50_RS26240 (LOY50_26185)	5866910..5867224	-	315	WP_024616743.1	XRE family transcriptional regulator	Antitoxin
LOY50_RS26245 (LOY50_26190)	5867217..5867582	-	366	WP_214509864.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY50_RS26250 (LOY50_26195)	5867946..5869628	+	1683	WP_258689544.1	NAD(P)/FAD-dependent oxidoreductase	-
LOY50_RS26255 (LOY50_26200)	5869641..5870435	+	795	WP_258689545.1	carbon-nitrogen hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14243.90 Da
• Isoelectric Point: 4.9750

>T225112 WP_214509864.1 NZ_CP087198:c5867582-5867217 [Pseudomonas sp. B21-010]
MKWDVEYTDEFELWWDRLSESEQDSVQVSVMLLGDTGPYLGFPHTSDIKGSRHGNLRELRVQHAGRPYRVLYAFDPRRCA
ILLIGGDKTGQDRWYQDYVPLAERLYDEHLEVLKREGFDHG

Nucleotide

• Length: 366 bp

>T225112 NZ_CP120971:c323624-323313 [Moellerella wisconsensis]
ATGCACGTAATTTCAAGAGATCCTTTTAACCAGGCATCCAAGCGATTTCCTAACAGTGCTCAGGCGTTATCTGACGTATA
TCGAACACTTAAGCGCGATAGTTATCAGACACCCGATGAGCTTAAGAAGGTTTTCCCCAGCCTTGACCGAATGAAGTACA
GAGAGAAATGGTGGGTTATCGATATCAGTGGAAATGCGTTAAGAATGATGTTTTTCGCCGACTTTGACCGGGGAAAAATA
TTCGTGAAACACATAACGACCCACGCAGAGTACGATAAGTTGACAGATCACTACCGGAGAACAAAAGCATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11899.64 Da
• Isoelectric Point: 9.6213

>AT225112 WP_024616743.1 NZ_CP087198:c5867224-5866910 [Pseudomonas sp. B21-010]
MAKKFSDLMARRSPDSQARAQALYQQLRAEMPLHELRQAQELSQEALAKALHINQAAVSKMERRTDMYISTLRNYIRAMG
GELEIIATFPEGQVRIENFSHPTP

Nucleotide

• Length: 315 bp

>AT225112 NZ_CP120971:c323316-322900 [Moellerella wisconsensis]
ATGATGTTTCAAGACGCCGTAAAAGCGGCACAAAACCTGGTAAATCTAGTCCCGTTACTGGGAGACAGCCATTCACGCCC
GGATTATGAAAAGGCCGTTCAGTTGGTGGAGCATCTTGTCGAGAACGACCCTGACAATCCGCTCATCGATATGATTTGCG
CAAAAATAGATGCGTATGAAAATGCCGCACCTGAATTTGCAGAGTTTAACCAACGTTTAGCAGAAAGTAATGATGGTGTG
GCAGCGTTAAGAACGCTGATGGATCAATACAACCTCAATACGACAGACTTTGGAAATGAGCTCGGTTCTCGTTCATACGT
CAGCCGTATCCTCAATGGGGAACGTGGATTAACCTTGGAGCACATCAAAAATCTGTCCAAACGCTTTGGTATTCCAGCCT
CAATATTTATTCGATGA

Structures

Antitoxin

• AlphaFold DB: A0A0F4XJJ2