Detailed information of TA system TA225101

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 5603406..5604061
• Replicon: chromosome
• Accession: NZ_CP087195
• Organism: Pseudomonas brassicacearum strain B21-016

Toxin (Protein)

• Gene name: higB
• Uniprot ID: G8QAX9
• Locus tag: LOY47_RS24795
• Protein ID: WP_013694185.1
• Coordinates: 5603717..5604061 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOY47_RS24790
• Protein ID: WP_003205391.1
• Coordinates: 5603406..5603720 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY47_RS24770 (LOY47_24745)	5599452..5600369	+	918	WP_003205387.1	LysR family transcriptional regulator	-
LOY47_RS24775 (LOY47_24750)	5600519..5601532	+	1014	WP_003205388.1	sensor domain-containing diguanylate cyclase	-
LOY47_RS24780 (LOY47_24755)	5601624..5602367	+	744	WP_013694184.1	DUF72 domain-containing protein	-
LOY47_RS24785 (LOY47_24760)	5602489..5603193	+	705	WP_003205390.1	hypothetical protein	-
LOY47_RS24790 (LOY47_24765)	5603406..5603720	-	315	WP_003205391.1	helix-turn-helix domain-containing protein	Antitoxin
LOY47_RS24795 (LOY47_24770)	5603717..5604061	-	345	WP_013694185.1	hypothetical protein	Toxin
LOY47_RS24800 (LOY47_24775)	5604201..5605088	-	888	WP_003205393.1	heme o synthase	-
LOY47_RS24805 (LOY47_24780)	5605100..5605435	-	336	WP_003205394.1	cytochrome o ubiquinol oxidase subunit IV	-
LOY47_RS24810 (LOY47_24785)	5605436..5606062	-	627	WP_003205395.1	cytochrome o ubiquinol oxidase subunit III	-
LOY47_RS24815 (LOY47_24790)	5606066..5608096	-	2031	WP_003205396.1	cytochrome o ubiquinol oxidase subunit I	-
LOY47_RS24820 (LOY47_24795)	5608100..5609047	-	948	WP_003205397.1	ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-87)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13753.72 Da
• Isoelectric Point: 8.3860

>T225101 WP_013694185.1 NZ_CP087195:c5604061-5603717 [Pseudomonas brassicacearum]
MRTLFFETTTFTDTVGHYLTDDEYRLLQSYMLQHPEVGDVMPRTGGFRKLRWFDERRGKGKRGGLRVIYYWLMNDRQFWM
FAIYDKDELENLTSEQEKTLKRAIEAELKVRGTL

Nucleotide

• Length: 345 bp

>T225101 NZ_CP120969:2024951-2025247 [Pseudomonas putida]
ATGGAAAAGAGAATGCCTAATTGCCCGCTGGAGCGGGTAAAAGCCCTGGCTGCGGCAAGGCGAATCCGCCCTACGGGTGC
TGCATTGAAAGGCGCCAAGGCGCTGGGAATGGATTTTCCGGGCATGCTTGAGGTGATCACCAGCTTGAAGCGCACTGATT
TCTACAAGAGCATGACCAGCCATATTGACCACCGGGTTTGGCAGGATGTGTATCGCCCGCTTACAGCAATTGGGTACGTT
TATTTGAAATTGTCCGTGGTAGACGACGTGCTCATCGTGTCTTTCAAGGAGTTGTAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11771.76 Da
• Isoelectric Point: 9.8409

>AT225101 WP_003205391.1 NZ_CP087195:c5603720-5603406 [Pseudomonas brassicacearum]
MKKRDLFAEMMQGVEEMAAHREGKITLRQISIEDKPVPEVSAQEIVALRDKLHMSQAVFAKSIRTSPGTLRNWEQEKSKP
NAQAALLIKLVEKFPDMVERLGAV

Nucleotide

• Length: 315 bp

>AT225101 NZ_CP120969:2025250-2025651 [Pseudomonas putida]
ATGAGATGTCCAATATGCGGCGGCTCGGAGCTTGCACCTGACATTCAGGGCATGCCCTATAGCTACAAAGGCGAGATGAC
AGTGATCCCTGAGGTAAGTGGCGATTACTGCTCCGCATGTGGTGAATGTGTACTGAGTCATGATGAAGCCATGCGCGTCA
GTCACTTGATGACGGCATTCGAGCGCCAGGTCAACGCAAATGTTGTGGATCCTTCCTTTATTGCCTCCATACGCAGAAAG
TTCGACCTCGACCAGCGTGAAGCGGGGGAAATCTTCGGTGGTGGGGTCAATGCGTTCTCCCGTTATGAAAACGGCAAGAC
CACACCGCCAGTGGCGTTGGTGAAACTGCTCAAGCTGCTGGATCGCCATCCAGAACTCTTTGAGGAAGTGCGTACTGCCT
GA

Structures

Toxin

• AlphaFold DB: G8QAX9