Detailed information of TA system TA022509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5283775..5284376
• Replicon: chromosome
• Accession: NC_009937
• Organism: Azorhizobium caulinodans ORS 571

Toxin (Protein)

• Gene name: graT
• Uniprot ID: A8HZZ5
• Locus tag: AZC_RS23800
• Protein ID: WP_012173165.1
• Coordinates: 5283775..5284059 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: AZC_RS23805
• Protein ID: WP_012173166.1
• Coordinates: 5284071..5284376 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZC_RS23775	5279364..5279798	-	435	WP_043879824.1	large conductance mechanosensitive channel protein MscL	-
AZC_RS23780	5280083..5280610	+	528	WP_043879825.1	3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA	-
AZC_RS23785	5280838..5282055	+	1218	WP_012173162.1	beta-ketoacyl-ACP synthase I	-
AZC_RS23790	5282170..5283027	+	858	WP_012173163.1	enoyl-ACP reductase FabI	-
AZC_RS23795	5283099..5283578	-	480	WP_012173164.1	transcription elongation factor GreA	-
AZC_RS23800	5283775..5284059	+	285	WP_012173165.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AZC_RS23805	5284071..5284376	+	306	WP_012173166.1	HigA family addiction module antidote protein	Antitoxin
AZC_RS23810	5284385..5286079	-	1695	WP_012173167.1	acyl-CoA dehydrogenase family protein	-
AZC_RS23815	5286191..5287306	-	1116	WP_052286055.1	FUSC family protein	-
AZC_RS23820	5287480..5288370	+	891	WP_043879826.1	YihY/virulence factor BrkB family protein	-
AZC_RS23825	5288370..5288846	+	477	WP_043879827.1	MmcB family DNA repair protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-73)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10583.08 Da
• Isoelectric Point: 8.0755

>T22509 WP_012173165.1 NC_009937:5283775-5284059 [Azorhizobium caulinodans ORS 571]
MIRSFRDRRAEAIFAGRHPGKGFPADLVRPAARKLAMLDAAAVLEDLRAPPANRLEELKGDRAGQHSIRVNDQFRICFVW
VDGGVEQVEITDYH

Nucleotide

• Length: 285 bp

>T22509 NC_009937:5283775-5284059 [Azorhizobium caulinodans ORS 571]
ATGATCCGGAGCTTCCGGGACCGCAGAGCCGAAGCGATCTTTGCGGGCCGCCATCCCGGCAAGGGATTTCCGGCCGATCT
GGTCCGACCCGCTGCCCGCAAGCTGGCGATGCTCGACGCGGCAGCGGTGTTGGAGGATTTGCGAGCCCCGCCAGCGAACC
GGCTGGAAGAGCTGAAGGGCGATCGGGCGGGCCAGCATTCCATTCGCGTGAACGACCAGTTCCGCATCTGCTTCGTCTGG
GTGGACGGCGGCGTGGAACAGGTGGAGATCACCGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11411.15 Da
• Isoelectric Point: 8.6585

>AT22509 WP_012173166.1 NC_009937:5284071-5284376 [Azorhizobium caulinodans ORS 571]
MPADEQKPLAPVHPGEILREEFLQPLGLTPYALGGALRVPRTRIERLVRGETSLTADTALRLARYFGTTPDFWLRLQTAH
DLRVEERRIAAELERIAPRAA

Nucleotide

• Length: 306 bp

>AT22509 NC_009937:5284071-5284376 [Azorhizobium caulinodans ORS 571]
ATGCCTGCCGATGAACAGAAACCCCTCGCGCCCGTGCATCCGGGCGAAATCCTGCGGGAAGAGTTCCTCCAGCCGCTCGG
CCTGACCCCCTACGCACTCGGGGGGGCACTCCGTGTGCCGCGCACGCGGATCGAGCGGCTCGTGCGCGGGGAAACCTCCC
TCACGGCGGACACCGCCCTGCGTCTTGCGCGCTATTTCGGCACCACGCCGGACTTCTGGCTGCGCCTTCAGACGGCGCAC
GATCTGCGCGTGGAGGAACGGCGGATCGCTGCCGAACTCGAACGCATCGCGCCGCGCGCCGCCTGA

Structures

Toxin

• AlphaFold DB: A8HZZ5