Detailed information of TA system TA225082

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4493208..4493862
• Replicon: chromosome
• Accession: NZ_CP087189
• Organism: Pseudomonas protegens strain B21-024

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4K9S3
• Locus tag: LOY29_RS20380
• Protein ID: WP_011062197.1
• Coordinates: 4493208..4493558 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOY29_RS20385
• Protein ID: WP_053152691.1
• Coordinates: 4493545..4493862 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY29_RS20370 (LOY29_20330)	4489967..4491499	+	1533	WP_019092840.1	NADH-quinone oxidoreductase subunit M	-
LOY29_RS20375 (LOY29_20335)	4491507..4492970	+	1464	WP_011062196.1	NADH-quinone oxidoreductase subunit NuoN	-
LOY29_RS20380 (LOY29_20340)	4493208..4493558	+	351	WP_011062197.1	hypothetical protein	Toxin
LOY29_RS20385 (LOY29_20345)	4493545..4493862	+	318	WP_053152691.1	transcriptional regulator	Antitoxin
LOY29_RS20390 (LOY29_20350)	4493931..4495313	-	1383	WP_015636301.1	cysteine--tRNA ligase	-
LOY29_RS20395 (LOY29_20355)	4495332..4497032	-	1701	WP_258610981.1	glutamine--tRNA ligase/YqeY domain fusion protein	-
LOY29_RS20400 (LOY29_20360)	4497303..4497806	+	504	WP_011062201.1	peptidylprolyl isomerase	-
LOY29_RS20405 (LOY29_20365)	4497803..4498555	+	753	WP_011062202.1	UDP-2,3-diacylglucosamine diphosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-101)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13922.05 Da
• Isoelectric Point: 9.6226

>T225082 WP_011062197.1 NZ_CP087189:4493208-4493558 [Pseudomonas protegens]
MNALFIELPAFARYRKGFLDDELFCELQIELLRHPHAGVLIQGTGGLRKMRFVDERRNKGRRGGLRVIYYWWSGGAQFWL
FTLFDKEQMDDLLPQQRQQLKQLLEREIEARRHDQT

Nucleotide

• Length: 351 bp

>T225082 NZ_CP120948:1498949-1499365 [Burkholderia contaminans]
GTGTATTTGATTGACACGAATATCATCAGCGAAATCAGGAAGGGCAAGCGAACCAACCGTGGCGTGCGGGCGTTTTTCAG
GCAGGCCGCGGCGGACGCAAGCCCGCTTTATCTTTCCGTCGTAACGGTTGCTGAACTTCGCCGCGGCGTCGATCTGATCC
GTCATCGTGGCGATCATCCCCAGGCGTCGGCGCTCGAGGCCTGGATGGCGACGATCCTGTCCGGCTATGCGCCGAACATC
CTGCCGGTCGACATCGAGATCAGCCAGATGTGGGGGCATTTGCGCGTGCCCGATCCGACCCACGAACTCGACAAGCTGAT
CGCGGCCACCGCGCTGATCAACGATCTCACGGTGGTCACGCGCAACGTCGAGGATTTCGCCCGCACCGGCGTCAAGCTGC
TGAACCCGTTCGACTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12050.92 Da
• Isoelectric Point: 10.4439

>AT225082 WP_053152691.1 NZ_CP087189:4493545-4493862 [Pseudomonas protegens]
MTKRDIFSELVEGIDALTQERQGKITLRSHKVKLDKLPPITAAEVMNIRQQLNLSRSVFAMYLRTNTRTLENWEQGRAIP
NAQATTLIRLVERYPDTVERLASLA

Nucleotide

• Length: 318 bp

>AT225082 NZ_CP120948:1498726-1498962 [Burkholderia contaminans]
ATGGCAAATCTACTGGTGCGTAACGTGGATGACAGTATCGTTCAGAGCCTGCGCGAGCAGGCTGCGGCAAATGGCAGGAG
TGCCGAGGCCGAACATCGGGCCATCCTGGCCGATGCGCTCGGCCGGCCGAAGCGGAAGACATTTGCGCAAGTGCTGATGA
GCATGCCCGATGTTGGCGACGACGCGGATTTTCAACGTGTTCAGGATTCCGGCGAGGCCAGGCGTGTATTTGATTGA

Structures

Toxin

• AlphaFold DB: Q4K9S3