Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 473768..474462 Replicon chromosome
Accession NZ_CP087187
Organism Pseudomonas atacamensis strain B21-026

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag LOY41_RS02090 Protein ID WP_258717413.1
Coordinates 473768..474064 (+) Length 99 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V8RDJ2
Locus tag LOY41_RS02095 Protein ID WP_024011299.1
Coordinates 474061..474462 (+) Length 134 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOY41_RS02055 (LOY41_02050) 469310..469855 - 546 WP_122705098.1 hypothetical protein -
LOY41_RS02060 (LOY41_02055) 469852..470106 - 255 WP_016772026.1 acyl carrier protein -
LOY41_RS02065 (LOY41_02060) 470116..470391 - 276 WP_039756723.1 phosphopantetheine-binding protein -
LOY41_RS02070 (LOY41_02065) 470369..471181 - 813 WP_122705096.1 lysophospholipid acyltransferase family protein -
LOY41_RS02075 (LOY41_02070) 471157..471882 - 726 WP_122705094.1 beta-ketoacyl synthase chain length factor -
LOY41_RS02080 (LOY41_02075) 472239..473009 + 771 WP_024011296.1 ParA family protein -
LOY41_RS02085 (LOY41_02080) 473140..473574 - 435 WP_024011297.1 thioredoxin TrxC -
LOY41_RS02090 (LOY41_02085) 473768..474064 + 297 WP_258717413.1 type II toxin-antitoxin system HigB family toxin Toxin
LOY41_RS02095 (LOY41_02090) 474061..474462 + 402 WP_024011299.1 transcriptional regulator Antitoxin
LOY41_RS02100 (LOY41_02095) 474469..475932 - 1464 WP_122705092.1 YdiU family protein -
LOY41_RS02105 (LOY41_02100) 475981..479334 - 3354 WP_258717414.1 mechanosensitive channel MscK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-90)

Antitoxin

Cro/C1-type helix-turn-helix domain

(78-132)


Sequences

Toxin        


Download         Length: 99 a.a.        Molecular weight: 11434.31 Da        Isoelectric Point: 10.6268

>T225062 WP_258717413.1 NZ_CP087187:473768-474064 [Pseudomonas atacamensis]
MHVFSEKPIREAKEKWPQSASALAQGFRLAKRSCPKDFSAMKSIFPAIDKVGQFHVFDLGGNKLRLIAFVSYRSQRLYIK
YVLDHREYDRGKWKEGKV

Download         Length: 297 bp

>T225062 NZ_CP120944:c2574626-2574477 [Escherichia coli O157:H7 str. EDL933]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin


Download         Length: 134 a.a.        Molecular weight: 15252.40 Da        Isoelectric Point: 4.4573

>AT225062 WP_024011299.1 NZ_CP087187:474061-474462 [Pseudomonas atacamensis]
MSILIKTVAEHWEYVAPLVRKPKSEEDYDALVRAVDEILELTGEDESHPLMSLLDIIGDWIEEWDRTHHPMAEASGEEVL
GYMMREHGLTQSDLPGVGTQSVVSEILSGKRKLNLRQIRWLAERFNVPIEVFI

Download         Length: 402 bp

>AT225062 NZ_CP120944:2574255-2574433 [Escherichia coli O157:H7 str. EDL933]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB V8RDJ2

References