Detailed information of TA system TA022506

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 973552..974156
• Replicon: chromosome
• Accession: NC_009937
• Organism: Azorhizobium caulinodans ORS 571

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A8HTU2
• Locus tag: AZC_RS04465
• Protein ID: WP_012169403.1
• Coordinates: 973875..974156 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AZC_RS04460
• Protein ID: WP_012169402.1
• Coordinates: 973552..973860 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AZC_RS24775	969386..970027	+	642	WP_012169397.1	SocA family protein	-
AZC_RS25400	970027..970437	+	411	WP_148209801.1	hypothetical protein	-
AZC_RS04440	971436..971825	-	390	WP_012169398.1	Arc family DNA-binding protein	-
AZC_RS24780	971923..972093	+	171	WP_081433897.1	Arc family DNA-binding protein	-
AZC_RS04445	972170..972916	+	747	WP_012169399.1	phage regulatory protein/antirepressor Ant	-
AZC_RS04450	972929..973273	+	345	WP_012169400.1	hypothetical protein	-
AZC_RS04460	973552..973860	-	309	WP_012169402.1	HigA family addiction module antidote protein	Antitoxin
AZC_RS04465	973875..974156	-	282	WP_012169403.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AZC_RS04470	975623..975862	-	240	WP_012169404.1	CsbD family protein	-
AZC_RS04475	975963..976805	-	843	WP_043878888.1	S1/P1 nuclease	-
AZC_RS04480	976822..977070	-	249	WP_012169406.1	hypothetical protein	-
AZC_RS04485	977248..977529	+	282	WP_043878889.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	932082..979305	47223

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (16-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-101)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10276.80 Da
• Isoelectric Point: 9.1493

>T22506 WP_012169403.1 NC_009937:c974156-973875 [Azorhizobium caulinodans ORS 571]
MIVSVRGKFAEAVVAGKAAKGFPAELLRAAERKLRMIHAATALEDLRSPPGNHLEALKADRAGQHSIRINDQWRVCFRWT
AAGAEDVEIVDYH

Nucleotide

• Length: 282 bp

>T22506 NC_009937:c974156-973875 [Azorhizobium caulinodans ORS 571]
ATGATCGTTTCCGTTCGAGGCAAGTTCGCCGAGGCGGTTGTGGCCGGCAAGGCTGCCAAGGGCTTTCCGGCGGAACTGCT
CAGAGCCGCCGAAAGGAAACTGCGCATGATCCACGCGGCGACTGCGCTGGAGGATCTTCGGTCTCCGCCCGGAAATCACC
TTGAGGCGCTGAAGGCGGATCGCGCGGGACAGCACTCCATCCGCATCAACGACCAGTGGCGCGTGTGCTTCCGTTGGACG
GCCGCCGGCGCCGAGGACGTTGAAATCGTCGATTACCACTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11649.38 Da
• Isoelectric Point: 5.7600

>AT22506 WP_012169402.1 NC_009937:c973860-973552 [Azorhizobium caulinodans ORS 571]
MAIKFARPVHPGEILREEYLVPLHLSAGQLAKALGVPRTRIERIVKEEFGISADTALRLARFFRTTPGFWLNLQQDYEIE
TESRKLAEELDRIQPFEEAAAA

Nucleotide

• Length: 309 bp

>AT22506 NC_009937:c973860-973552 [Azorhizobium caulinodans ORS 571]
ATGGCCATCAAGTTCGCTCGCCCTGTCCACCCGGGCGAGATCCTGCGCGAGGAATATTTGGTCCCGCTGCACTTGAGCGC
CGGCCAACTCGCCAAGGCCCTGGGCGTGCCGCGCACGCGCATCGAGCGGATCGTGAAGGAGGAGTTCGGCATCTCCGCCG
ACACCGCGCTCCGGCTTGCCCGATTTTTCAGGACGACGCCCGGGTTCTGGCTGAACCTCCAGCAGGATTACGAGATCGAG
ACGGAGAGCAGGAAACTGGCGGAAGAGCTGGATCGCATCCAGCCGTTTGAGGAAGCCGCCGCAGCGTGA

Structures

Toxin

• AlphaFold DB: A8HTU2