Detailed information of TA system TA225057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5088344..5088924
• Replicon: chromosome
• Accession: NZ_CP087186
• Organism: Pseudomonas siliginis strain B21-063

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A554AH63
• Locus tag: LOY48_RS22425
• Protein ID: WP_041477766.1
• Coordinates: 5088344..5088622 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOY48_RS22430
• Protein ID: WP_016773085.1
• Coordinates: 5088637..5088924 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY48_RS22405 (LOY48_22370)	5083829..5083996	-	168	WP_016773090.1	DUF2474 domain-containing protein	-
LOY48_RS22410 (LOY48_22375)	5084081..5085088	-	1008	WP_016773089.1	cytochrome d ubiquinol oxidase subunit II	-
LOY48_RS22415 (LOY48_22380)	5085092..5086537	-	1446	WP_041477768.1	cytochrome ubiquinol oxidase subunit I	-
LOY48_RS22420 (LOY48_22385)	5087008..5088243	+	1236	WP_258707222.1	MFS transporter	-
LOY48_RS22425 (LOY48_22390)	5088344..5088622	+	279	WP_041477766.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY48_RS22430 (LOY48_22395)	5088637..5088924	+	288	WP_016773085.1	HigA family addiction module antitoxin	Antitoxin
LOY48_RS22435 (LOY48_22400)	5088929..5089480	-	552	WP_150742273.1	DJ-1/PfpI family protein	-
LOY48_RS22440 (LOY48_22405)	5089548..5089994	-	447	WP_150742274.1	DUF4879 domain-containing protein	-
LOY48_RS22445 (LOY48_22410)	5090215..5091510	+	1296	WP_258707223.1	NCS2 family permease	-
LOY48_RS22450 (LOY48_22415)	5091507..5092586	+	1080	WP_150742275.1	tRNA (uridine(54)-C5)-methyltransferase TrmA	-
LOY48_RS22455 (LOY48_22420)	5092857..5093297	+	441	WP_137215639.1	type II 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-75)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10771.34 Da
• Isoelectric Point: 9.5056

>T225057 WP_041477766.1 NZ_CP087186:5088344-5088622 [Pseudomonas siliginis]
MIKSFQHKGLRGFYETGTTRGIRADHAKRLSRMLQFMDRATLPADLDLPGWRLHPLKGELSEYWSLSVSGNWRVIFRFVG
SDIELVDYLDYH

Nucleotide

• Length: 279 bp

>T225057 NZ_CP120944:695876-696190 [Escherichia coli O157:H7 str. EDL933]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10525.13 Da
• Isoelectric Point: 6.6962

>AT225057 WP_016773085.1 NZ_CP087186:5088637-5088924 [Pseudomonas siliginis]
MPMHNPPHPGETLLLDVLPELGISVSELARHLGFARPHLSRVLHGHAPISPDLAVRLERAGIGKARVWLGVQTDYDLWQA
EHREQPMIEPLAAHG

Nucleotide

• Length: 288 bp

>AT225057 NZ_CP120944:696187-696603 [Escherichia coli O157:H7 str. EDL933]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A554AH63