Detailed information of TA system TA225056

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4813059..4813714
• Replicon: chromosome
• Accession: NZ_CP087186
• Organism: Pseudomonas siliginis strain B21-063

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LOY48_RS21090
• Protein ID: WP_258707171.1
• Coordinates: 4813370..4813714 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOY48_RS21085
• Protein ID: WP_041479944.1
• Coordinates: 4813059..4813373 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY48_RS21065 (LOY48_21040)	4809087..4810259	-	1173	WP_258707169.1	aspartate aminotransferase family protein	-
LOY48_RS21070 (LOY48_21045)	4810362..4811288	+	927	WP_252882652.1	LysR family transcriptional regulator	-
LOY48_RS21075 (LOY48_21050)	4811423..4812124	+	702	WP_258707170.1	hypothetical protein	-
LOY48_RS21080 (LOY48_21055)	4812381..4812872	-	492	WP_016773306.1	RidA family protein	-
LOY48_RS21085 (LOY48_21060)	4813059..4813373	-	315	WP_041479944.1	helix-turn-helix domain-containing protein	Antitoxin
LOY48_RS21090 (LOY48_21065)	4813370..4813714	-	345	WP_258707171.1	toxin	Toxin
LOY48_RS21095 (LOY48_21070)	4813830..4814717	-	888	WP_016773305.1	heme o synthase	-
LOY48_RS21100 (LOY48_21075)	4814728..4815063	-	336	WP_011335842.1	cytochrome o ubiquinol oxidase subunit IV	-
LOY48_RS21105 (LOY48_21080)	4815063..4815686	-	624	WP_024014230.1	cytochrome o ubiquinol oxidase subunit III	-
LOY48_RS21110 (LOY48_21085)	4815690..4817720	-	2031	WP_041479948.1	cytochrome o ubiquinol oxidase subunit I	-
LOY48_RS21115 (LOY48_21090)	4817724..4818665	-	942	WP_024014231.1	ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (42-94)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13505.45 Da
• Isoelectric Point: 10.3452

>T225056 WP_258707171.1 NZ_CP087186:c4813714-4813370 [Pseudomonas siliginis]
MRTIFFETTIFTKSVGRYLTDDEYRTLQNYLQSNPLAGDVMPRTGGFRKLRWADSRRGKGRRGGLRVLYYWLMNDGQFWL
FAIYDKDELEKLTAEQERALRTAIDKELKKRGTP

Nucleotide

• Length: 345 bp

>T225056 NZ_CP120944:c653390-652926 [Escherichia coli O157:H7 str. EDL933]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11900.82 Da
• Isoelectric Point: 9.9443

>AT225056 WP_041479944.1 NZ_CP087186:c4813373-4813059 [Pseudomonas siliginis]
MKKRDLFAELMQGVDEMAAQREGKITLRNTVLEDKPVPEVGAQEIVALREKLHMSQAVFASRIRTSPNTLRNWEQEKSKP
NAQAALLIRLVEKFPDMVERLNTV

Nucleotide

• Length: 315 bp

>AT225056 NZ_CP120944:c653725-653390 [Escherichia coli O157:H7 str. EDL933]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAGCAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATACAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA