Detailed information of TA system TA022504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 6281710..6282322
• Replicon: chromosome
• Accession: NC_009925
• Organism: Acaryochloris marina MBIC11017

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B0C629
• Locus tag: AM1_RS28025
• Protein ID: WP_012166333.1
• Coordinates: 6281710..6282042 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AM1_RS28030
• Protein ID: WP_012166334.1
• Coordinates: 6282032..6282322 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AM1_RS28010	6276955..6277962	-	1008	WP_012166331.1	tyrosine-type recombinase/integrase	-
AM1_RS28015	6278208..6279311	+	1104	WP_012166332.1	potassium channel protein	-
AM1_RS28020	6279564..6280808	-	1245	WP_012160803.1	ISL3-like element ISAcma10 family transposase	-
AM1_RS41350	6281364..6281495	+	132	WP_148215983.1	DUF4351 domain-containing protein	-
AM1_RS28025	6281710..6282042	+	333	WP_012166333.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AM1_RS28030	6282032..6282322	+	291	WP_012166334.1	helix-turn-helix domain-containing protein	Antitoxin
AM1_RS28035	6282469..6283389	-	921	WP_041661100.1	DUF3365 domain-containing protein	-
AM1_RS28040	6284168..6285289	-	1122	WP_049783802.1	calcium-binding protein	-
AM1_RS28045	6285564..6286601	-	1038	WP_148215984.1	calcium-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	6279564..6280808	1244

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-89)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12742.93 Da
• Isoelectric Point: 9.8173

>T22504 WP_012166333.1 NC_009925:6281710-6282042 [Acaryochloris marina MBIC11017]
MAWSVQYYSETVESEVLALPPGLLARYLRLTDLMLEFGPNLGMPHTQSMGDRLFELRVKSKEGIARVFYCTLIGERIVML
HSFVKKTQKTPNRELKIARRRLKEVIDNDS

Nucleotide

• Length: 333 bp

>T22504 NC_009925:6281710-6282042 [Acaryochloris marina MBIC11017]
ATGGCCTGGAGTGTTCAATATTATTCCGAAACAGTTGAATCTGAGGTACTTGCTCTGCCTCCAGGTTTGTTAGCTAGATA
TTTGAGGCTCACCGACCTCATGTTAGAGTTTGGTCCCAATCTGGGGATGCCTCACACCCAATCCATGGGCGATCGCCTAT
TTGAATTGAGGGTAAAGAGCAAGGAAGGGATTGCGAGGGTGTTTTATTGCACCCTGATCGGCGAAAGGATAGTGATGCTG
CACAGCTTTGTGAAGAAGACTCAAAAGACCCCCAATCGTGAACTCAAGATCGCGCGTCGTCGGTTAAAAGAGGTGATAGA
CAATGACTCATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10587.10 Da
• Isoelectric Point: 7.1668

>AT22504 WP_012166334.1 NC_009925:6282032-6282322 [Acaryochloris marina MBIC11017]
MTHEDLKAKALNNPEVAEEYEKLEEEFSLLRQMLAARQNAGLTQADIAEKMGTKATAVTRLESSLSSGKHSPSLSTLRKY
AKAVGCRLEVKLVPEQ

Nucleotide

• Length: 291 bp

>AT22504 NC_009925:6282032-6282322 [Acaryochloris marina MBIC11017]
ATGACTCATGAGGATCTCAAAGCAAAAGCCCTAAACAATCCAGAAGTGGCTGAAGAGTACGAAAAACTAGAGGAAGAGTT
TAGTCTTTTAAGGCAAATGCTTGCTGCCCGCCAGAATGCAGGTCTAACTCAAGCTGACATTGCTGAAAAAATGGGGACAA
AGGCAACAGCAGTGACCCGGCTGGAATCATCCCTCAGTAGTGGTAAGCATTCACCATCCCTATCCACTTTGCGAAAGTAT
GCCAAGGCAGTGGGTTGCCGTTTAGAAGTGAAGTTAGTGCCAGAACAGTAG

Structures

Toxin

• AlphaFold DB: B0C629