Detailed information of TA system TA225010

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 292920..293524
• Replicon: chromosome
• Accession: NZ_CP087177
• Organism: Pseudomonas moraviensis strain B21-039

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LOY57_RS01260
• Protein ID: WP_064363397.1
• Coordinates: 293210..293524 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1N7CI26
• Locus tag: LOY57_RS01255
• Protein ID: WP_064363398.1
• Coordinates: 292920..293207 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY57_RS01235 (LOY57_01235)	288695..289099	+	405	WP_064363401.1	RNA-binding S4 domain-containing protein	-
LOY57_RS01240 (LOY57_01240)	289258..290061	-	804	WP_064363400.1	phosphatase PAP2 family protein	-
LOY57_RS01245 (LOY57_01245)	290168..291070	+	903	WP_042608585.1	Hsp33 family molecular chaperone HslO	-
LOY57_RS01250 (LOY57_01250)	291252..292793	+	1542	WP_016772179.1	phosphoenolpyruvate carboxykinase	-
LOY57_RS01255 (LOY57_01255)	292920..293207	-	288	WP_064363398.1	NadS family protein	Antitoxin
LOY57_RS01260 (LOY57_01260)	293210..293524	-	315	WP_064363397.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY57_RS01265 (LOY57_01265)	293630..293929	-	300	WP_064363396.1	hypothetical protein	-
LOY57_RS01270 (LOY57_01270)	294275..295417	-	1143	WP_064363395.1	hypothetical protein	-
LOY57_RS01275 (LOY57_01275)	295716..297650	+	1935	WP_258640035.1	ATP-dependent DNA helicase RecQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-73)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11614.29 Da
• Isoelectric Point: 9.8054

>T225010 WP_064363397.1 NZ_CP087177:c293524-293210 [Pseudomonas moraviensis]
MIFIETSVFTRRVRELLDDDAYATFQKQLVADPSIGDVIEGTGGVRKARVASKGHGKRGGAGVIYYHFVSASQIALLMIY
SKNEQQDLTHDERKALKAMIGNWR

Nucleotide

• Length: 315 bp

>T225010 NZ_CP120837:200449-200934 [Salmonella enterica subsp. enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGTGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10727.38 Da
• Isoelectric Point: 8.7453

>AT225010 WP_064363398.1 NZ_CP087177:c293207-292920 [Pseudomonas moraviensis]
MSKFFDELMESVQEMDEILRGEKKPSREFHVDALQVKQIRKATGMTQAKFAALIDVQLGTLRNWEQGRRQPTGPAKALLK
AIHNDPVHVLSALAD

Nucleotide

• Length: 288 bp

>AT225010 NZ_CP120837:200174-200461 [Salmonella enterica subsp. enterica]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCTGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCAGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1N7CI26