Detailed information of TA system TA224993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5115631..5116211
• Replicon: chromosome
• Accession: NZ_CP087171
• Organism: Pseudomonas atacamensis strain B21-045

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1E8P922
• Locus tag: LOY27_RS22940
• Protein ID: WP_038861219.1
• Coordinates: 5115631..5115909 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOY27_RS22945
• Protein ID: WP_016773085.1
• Coordinates: 5115924..5116211 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY27_RS22920 (LOY27_22880)	5111113..5111280	-	168	WP_016773090.1	DUF2474 domain-containing protein	-
LOY27_RS22925 (LOY27_22885)	5111365..5112372	-	1008	WP_024014408.1	cytochrome d ubiquinol oxidase subunit II	-
LOY27_RS22930 (LOY27_22890)	5112376..5113821	-	1446	WP_024014409.1	cytochrome ubiquinol oxidase subunit I	-
LOY27_RS22935 (LOY27_22895)	5114295..5115530	+	1236	WP_016773087.1	MFS transporter	-
LOY27_RS22940 (LOY27_22900)	5115631..5115909	+	279	WP_038861219.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY27_RS22945 (LOY27_22905)	5115924..5116211	+	288	WP_016773085.1	HigA family addiction module antitoxin	Antitoxin
LOY27_RS22950 (LOY27_22910)	5116216..5116767	-	552	WP_258710562.1	DJ-1/PfpI family protein	-
LOY27_RS22955 (LOY27_22915)	5116834..5117280	-	447	WP_258711707.1	YolA family protein	-
LOY27_RS22960 (LOY27_22920)	5117501..5118796	+	1296	WP_016773083.1	NCS2 family permease	-
LOY27_RS22965 (LOY27_22925)	5118793..5119872	+	1080	WP_258710563.1	tRNA (uridine(54)-C5)-methyltransferase TrmA	-
LOY27_RS22970 (LOY27_22930)	5120067..5120507	+	441	WP_258710564.1	type II 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-75)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10757.32 Da
• Isoelectric Point: 9.5056

>T224993 WP_038861219.1 NZ_CP087171:5115631-5115909 [Pseudomonas atacamensis]
MIKSFQHKGLRGFYETGTTRGIRADHAKRLSRMLQFMDRATLPGDLDLPGWRLHPLKGELSEYWSLSVSGNWRVIFRFVG
SDIELVDYLDYH

Nucleotide

• Length: 279 bp

>T224993 NZ_CP120718:c5903330-5902980 [Paenibacillus sp. BR1-192]
GTGATCGTTAAACGGGGCGACGTATTTTTTGCGGATTTATCACCTGTTGTCGGTTCTGAGCAAGGCGGCGTGAGACCGGT
CCTTGTCATTCAGAACGATATCGGCAACCGGTTTAGTCCGACTGTGATTGTTGCAGCCATTACGGCACAGATTCAAAAAG
CCAAGCTGCCGACTCACGTCGAAATCGACGCGGCGTCGCATGGATTTGACCGGGATTCCGTCATTTTGCTAGAGCAGATC
CGGACGATTGATAAGCAGCGGCTAACCGACAAAATCACCCATCTTGATGATGAAATGATGAGCAAGGTGGATGATTCCCT
GCAAATAAGTCTGGGCTTGATTGATTTTTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10525.13 Da
• Isoelectric Point: 6.6962

>AT224993 WP_016773085.1 NZ_CP087171:5115924-5116211 [Pseudomonas atacamensis]
MPMHNPPHPGETLLLDVLPELGISVSELARHLGFARPHLSRVLHGHAPISPDLAVRLERAGIGKARVWLGVQTDYDLWQA
EHREQPMIEPLAAHG

Nucleotide

• Length: 288 bp

>AT224993 NZ_CP120718:c5903615-5903334 [Paenibacillus sp. BR1-192]
GTGGCCAATATGCAGAACACCAAACGAATCATGATCAGTTTACCGGATCATCTTTTGCAGGAAGTGGACGGGATCGCTCA
GCTGGAAAATTCCAACCGCAGTGAGCTTATTAGGCAGGCCATGAAGTTGTACCTGAATGAACGGAAGAAACGTTACATTC
GTGAATCCATGCAGCGGGGTTATATGGAAATGGCCAAAATTAACCTGACCATGGCTTCTGAGGCGTTTCACGCGGAGGAA
GATGCAGACAACACTCTAGACCGCTTAGTAAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A1E8P922