Detailed information of TA system TA224984

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 3602430..3603088
• Replicon: chromosome
• Accession: NZ_CP087170
• Organism: Pseudomonas rhodesiae strain B21-046

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LOY39_RS16120
• Protein ID: WP_034099048.1
• Coordinates: 3602744..3603088 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOY39_RS16115
• Protein ID: WP_258694809.1
• Coordinates: 3602430..3602747 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY39_RS16095 (LOY39_16075)	3600343..3601194	-	852	WP_034099041.1	hypothetical protein	-
LOY39_RS16100 (LOY39_16080)	3601414..3601533	+	120	Protein_3171	IS5/IS1182 family transposase	-
LOY39_RS16105 (LOY39_16085)	3601618..3601854	-	237	WP_034099043.1	YdaS family helix-turn-helix protein	-
LOY39_RS16110 (LOY39_16090)	3601953..3602378	+	426	WP_005789662.1	helix-turn-helix domain-containing protein	-
LOY39_RS16115 (LOY39_16095)	3602430..3602747	-	318	WP_258694809.1	transcriptional regulator	Antitoxin
LOY39_RS16120 (LOY39_16100)	3602744..3603088	-	345	WP_034099048.1	toxin	Toxin
LOY39_RS16125 (LOY39_16105)	3603211..3604014	-	804	WP_034099050.1	phage antirepressor KilAC domain-containing protein	-
LOY39_RS16130 (LOY39_16110)	3604011..3604322	-	312	WP_258694810.1	DUF3077 domain-containing protein	-
LOY39_RS16135 (LOY39_16115)	3604396..3604551	-	156	WP_258694811.1	Arc family DNA-binding protein	-
LOY39_RS16140 (LOY39_16120)	3604696..3605082	+	387	WP_258694812.1	helix-turn-helix transcriptional regulator	-
LOY39_RS16145 (LOY39_16125)	3605332..3606249	-	918	WP_258694813.1	hypothetical protein	-
LOY39_RS16150 (LOY39_16130)	3606450..3606923	+	474	WP_034099054.1	hypothetical protein	-
LOY39_RS16155 (LOY39_16135)	3607029..3607316	+	288	WP_034099056.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
LOY39_RS16160 (LOY39_16140)	3607306..3607593	+	288	WP_034099058.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3595243..3612752	17509
	-	flank	IS/Tn	-	-	3601414..3601536	122

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (46-95)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13141.93 Da
• Isoelectric Point: 9.6563

>T224984 WP_034099048.1 NZ_CP087170:c3603088-3602744 [Pseudomonas rhodesiae]
MKTVFFETTSFTATVGDYLTDDEYRQLQTEMQANPEAGDVMPRTGGFRKLRWQDTRRGKGKRGGLRVIYYWLLNDGQFWM
FSIYDKDEMANLTAAQEKALKAAIDAELKKRGGK

Nucleotide

• Length: 345 bp

>T224984 NZ_CP120681:1638324-1638440 [Bacillus subtilis]
ATGACTGTTTACGAATCATTAATGATAATGATCAATTTTGGCGGATTGATATTAAATACCGTCTTGTTGATCTTCAATAT
AATGATGATTGTAACGTCAAGCCAAAAGAAAAAATAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12056.96 Da
• Isoelectric Point: 10.1426

>AT224984 WP_258694809.1 NZ_CP087170:c3602747-3602430 [Pseudomonas rhodesiae]
MKKKRDLFAELMQGVNEMGEHRQGKITLRQYEVEALSPPEVTAAEIVSIRENLHMSQPVFARQIRTSPDTLKNWEQSKSK
PNAQAALLIKLVQRFPDMVERLNAV

Nucleotide

• Length: 318 bp

>AT224984 NZ_CP120681:c1638614-1638435 [Bacillus subtilis]
AATGATACAATTATATAATTATTTTTTGCATTTTGTTTAATTAAATGCATAAAATAAAAAGACCAGGGTGTTGGTAGCAC
CCTGGCTCTGTACAAAAGCTGCCCATCAAAGGGCTTGCTCGTTGTCAAATCTGCGTAGGCCTAACCCTTCAGGTGTCCAA
ACTCAAGGGAAGGTCTATTT