Detailed information of TA system TA224972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5713009..5713600
• Replicon: chromosome
• Accession: NZ_CP087167
• Organism: Pseudomonas atacamensis strain B21-050

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LOY49_RS25770
• Protein ID: WP_082228595.1
• Coordinates: 5713322..5713600 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: A0A341FAY9
• Locus tag: LOY49_RS25765
• Protein ID: WP_051600808.1
• Coordinates: 5713009..5713314 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY49_RS25735 (LOY49_25705)	5708561..5709268	+	708	WP_071172133.1	HAD-IA family hydrolase	-
LOY49_RS25740 (LOY49_25710)	5709458..5709784	-	327	WP_016772583.1	transcriptional regulator SutA	-
LOY49_RS25745 (LOY49_25715)	5709886..5710311	-	426	WP_024014775.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
LOY49_RS25750 (LOY49_25720)	5710527..5711864	-	1338	WP_041476939.1	ammonium transporter	-
LOY49_RS25755 (LOY49_25725)	5711902..5712240	-	339	WP_002555808.1	P-II family nitrogen regulator	-
LOY49_RS25760 (LOY49_25730)	5712635..5712895	+	261	WP_122603430.1	accessory factor UbiK family protein	-
LOY49_RS25765 (LOY49_25735)	5713009..5713314	-	306	WP_051600808.1	HigA family addiction module antitoxin	Antitoxin
LOY49_RS25770 (LOY49_25740)	5713322..5713600	-	279	WP_082228595.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY49_RS25775 (LOY49_25745)	5713926..5715419	+	1494	WP_258604135.1	YifB family Mg chelatase-like AAA ATPase	-
LOY49_RS25780 (LOY49_25750)	5715416..5716630	-	1215	WP_258604137.1	aldose 1-epimerase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10401.95 Da
• Isoelectric Point: 7.7181

>T224972 WP_082228595.1 NZ_CP087167:c5713600-5713322 [Pseudomonas atacamensis]
MIVSFRCPETEYLFRSGKTRFWSAILSVAERKLTMLDAAAALADLRSPPGNRLESLGGDRKGQCSIRINAQWRICFVWGL
NGPEDVEIVDYH

Nucleotide

• Length: 279 bp

>T224972 NZ_CP120671:c2927688-2927425 [Salmonella enterica]
ATGAATGCCTCCGGCGCCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCACCTGTTACTCACAAATCCCCAGCGG
CGACTGCCAGGAAGAAGCGGGATTTGAGACCGGGGCGAGCGTTGTTGTGAAGATCTCCGAGCGGGGTTTGATCCTGATTG
CAGAAACGGATGAAGTGAGGGATCTACGGAAGGAGCTTTATCAGGTGAAAAAGTCGATGAAGCATATTAAGGCGGGGGTA
AATAATGTGGTGAACGGGAATTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11232.97 Da
• Isoelectric Point: 6.4556

>AT224972 WP_051600808.1 NZ_CP087167:c5713314-5713009 [Pseudomonas atacamensis]
MTKNGMRPVHPGEILKEEYLEPLSLTAAALARALSVSTPTVNDIVLQRRGISADMAIRLSICLDTTPEFWLNLQSTYDLR
KAEIERGAAIRDQVERLAHCA

Nucleotide

• Length: 306 bp

>AT224972 NZ_CP120671:2927435-2927845 [Salmonella enterica]
TTCACCACATTATTTACCCCCGCCTTAATATGCTTCATCGACTTTTTCACCTGATAAAGCTCCTTCCGTAGATCCCTCAC
TTCATCCGTTTCTGCAATCAGGATCAAACCCCGCTCGGAGATCTTCACAACAACGCTCGCCCCGGTCTCAAATCCCGCTT
CTTCCTGGCAGTCGCCGCTGGGGATTTGTGAGTAACAGGTGGTCATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGGCG
CCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTGAG
CGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCCGG
GGCCGTATTTT

Structures

Antitoxin

• AlphaFold DB: A0A341FAY9