Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 5089624..5090204 Replicon chromosome
Accession NZ_CP087167
Organism Pseudomonas atacamensis strain B21-050

Toxin (Protein)


Gene name higB Uniprot ID A0A1E8P922
Locus tag LOY49_RS22795 Protein ID WP_038861219.1
Coordinates 5089624..5089902 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LOY49_RS22800 Protein ID WP_016773085.1
Coordinates 5089917..5090204 (+) Length 96 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOY49_RS22775 (LOY49_22745) 5085116..5085283 - 168 WP_016773090.1 DUF2474 domain-containing protein -
LOY49_RS22780 (LOY49_22750) 5085361..5086368 - 1008 WP_024014408.1 cytochrome d ubiquinol oxidase subunit II -
LOY49_RS22785 (LOY49_22755) 5086372..5087817 - 1446 WP_024014409.1 cytochrome ubiquinol oxidase subunit I -
LOY49_RS22790 (LOY49_22760) 5088288..5089523 + 1236 WP_016773087.1 MFS transporter -
LOY49_RS22795 (LOY49_22765) 5089624..5089902 + 279 WP_038861219.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LOY49_RS22800 (LOY49_22770) 5089917..5090204 + 288 WP_016773085.1 HigA family addiction module antitoxin Antitoxin
LOY49_RS22805 (LOY49_22775) 5090209..5090760 - 552 WP_016773084.1 DJ-1/PfpI family protein -
LOY49_RS22810 (LOY49_22780) 5090828..5091274 - 447 WP_206422160.1 DUF4879 domain-containing protein -
LOY49_RS22815 (LOY49_22785) 5091495..5092790 + 1296 WP_016773083.1 NCS2 family permease -
LOY49_RS22820 (LOY49_22790) 5092787..5093866 + 1080 WP_258603727.1 tRNA (uridine(54)-C5)-methyltransferase TrmA -
LOY49_RS22825 (LOY49_22795) 5094061..5094501 + 441 WP_137215639.1 type II 3-dehydroquinate dehydratase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(3-92)

Antitoxin

(20-75)


Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10757.32 Da        Isoelectric Point: 9.5056

>T224971 WP_038861219.1 NZ_CP087167:5089624-5089902 [Pseudomonas atacamensis]
MIKSFQHKGLRGFYETGTTRGIRADHAKRLSRMLQFMDRATLPGDLDLPGWRLHPLKGELSEYWSLSVSGNWRVIFRFVG
SDIELVDYLDYH

Download         Length: 279 bp

>T224971 NZ_CP120671:2409136-2409420 [Salmonella enterica]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGAAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGGTCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGTCTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10525.13 Da        Isoelectric Point: 6.6962

>AT224971 WP_016773085.1 NZ_CP087167:5089917-5090204 [Pseudomonas atacamensis]
MPMHNPPHPGETLLLDVLPELGISVSELARHLGFARPHLSRVLHGHAPISPDLAVRLERAGIGKARVWLGVQTDYDLWQA
EHREQPMIEPLAAHG

Download         Length: 288 bp

>AT224971 NZ_CP120671:2408904-2409146 [Salmonella enterica]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A1E8P922


Antitoxin

Source ID Structure

References