Detailed information of TA system TA224963

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 284716..285320
• Replicon: chromosome
• Accession: NZ_CP087167
• Organism: Pseudomonas atacamensis strain B21-050

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LOY49_RS01215
• Protein ID: WP_130910162.1
• Coordinates: 285006..285320 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOY49_RS01210
• Protein ID: WP_016772178.1
• Coordinates: 284716..285003 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY49_RS01190 (LOY49_01190)	280582..280986	+	405	WP_122611135.1	S4 domain-containing protein	-
LOY49_RS01195 (LOY49_01195)	281076..281876	-	801	WP_122611134.1	phosphatase PAP2 family protein	-
LOY49_RS01200 (LOY49_01200)	281984..282886	+	903	WP_258604472.1	Hsp33 family molecular chaperone HslO	-
LOY49_RS01205 (LOY49_01205)	283068..284609	+	1542	WP_016772179.1	phosphoenolpyruvate carboxykinase	-
LOY49_RS01210 (LOY49_01210)	284716..285003	-	288	WP_016772178.1	NadS family protein	Antitoxin
LOY49_RS01215 (LOY49_01215)	285006..285320	-	315	WP_130910162.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY49_RS01220 (LOY49_01220)	285657..286799	-	1143	WP_258604475.1	hypothetical protein	-
LOY49_RS01225 (LOY49_01225)	287073..289007	+	1935	WP_160768534.1	ATP-dependent DNA helicase RecQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11722.48 Da
• Isoelectric Point: 10.2637

>T224963 WP_130910162.1 NZ_CP087167:c285320-285006 [Pseudomonas atacamensis]
MIFIETSVFTRRVRELLDDDAYATFQKQLVANPSIGDVIEGTGGVRKARVASKGHGKRGGARVIYYHFVSASQIALLMIY
PKNEQQDLTHDERKALKAMIGNWR

Nucleotide

• Length: 315 bp

>T224963 NZ_CP120671:434851-435378 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGCGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10753.42 Da
• Isoelectric Point: 8.6214

>AT224963 WP_016772178.1 NZ_CP087167:c285003-284716 [Pseudomonas atacamensis]
MSKFFDELMESVQEMDEILRGEKKPSREFYVDALQVKQIRKATGMTQAKFAALIDVQLGTLRNWEQGRRQPTGPAKALLK
AIHNDPVHVLSALAD

Nucleotide

• Length: 288 bp

>AT224963 NZ_CP120671:434564-434854 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGACTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA