Detailed information of TA system TA224951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1121966..1122570
• Replicon: chromosome
• Accession: NZ_CP087165
• Organism: Pseudomonas sichuanensis strain B21-052

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LOY46_RS05305
• Protein ID: WP_125462793.1
• Coordinates: 1122250..1122570 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOY46_RS05300
• Protein ID: WP_125462792.1
• Coordinates: 1121966..1122253 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOY46_RS05275 (LOY46_05275)	1117944..1118552	+	609	WP_125462787.1	hypothetical protein	-
LOY46_RS05280 (LOY46_05280)	1118652..1119872	+	1221	WP_258705788.1	SAM-dependent methyltransferase	-
LOY46_RS05285 (LOY46_05285)	1120035..1120313	+	279	WP_125462789.1	hypothetical protein	-
LOY46_RS05290 (LOY46_05290)	1120316..1120825	+	510	WP_125462790.1	DNA-binding protein	-
LOY46_RS05295 (LOY46_05295)	1120848..1121858	-	1011	WP_258705789.1	DUF4917 family protein	-
LOY46_RS05300 (LOY46_05300)	1121966..1122253	-	288	WP_125462792.1	NadS family protein	Antitoxin
LOY46_RS05305 (LOY46_05305)	1122250..1122570	-	321	WP_125462793.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LOY46_RS05310 (LOY46_05310)	1122702..1124402	-	1701	WP_125462794.1	SulP family inorganic anion transporter	-
LOY46_RS05320 (LOY46_05320)	1124795..1125679	-	885	WP_125462795.1	alpha/beta hydrolase	-
LOY46_RS05325 (LOY46_05325)	1126127..1126819	-	693	WP_125462796.1	16S rRNA pseudouridine(516) synthase	-
LOY46_RS05330 (LOY46_05330)	1126851..1127072	-	222	WP_125462797.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-87)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12091.90 Da
• Isoelectric Point: 10.1563

>T224951 WP_125462793.1 NZ_CP087165:c1122570-1122250 [Pseudomonas sichuanensis]
MLFTETAVFTKRAKELLDDETYRLLQVRLLISPDAGDVIEGTGGLRKIRAPANGHGKRGGARVIYYHFTARSHITLLYIF
AKNEQTELSAEQRKALKSVIENWRYP

Nucleotide

• Length: 321 bp

>T224951 NZ_CP120638:787750-788031 [Rhizobium sp. 32-5/1]
ATGCGCTATAAACTTGCCTTCCTCGCAGCGGCGCTGAAGGAATGGGAGAAACTGGATAGCAATACGCGAGCGCTGTTCAA
GAAGAGGCTCACGGAGCGATTGGAAACACCGCGGGCTCCTTCGGCGCAGTTGCATGGTTCCAAGGACCGCTACAAGATCA
AGCTACGCAGCGCCGGTTATCGCCTCGTCTATGAAGTCCGCGATCAGGACATCGTCGTTGTCGTCATCGCCGTTGGCCGA
CGGGATCACAGCGATATCTATGGCAAGGCGGCAAAAAGGTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10949.55 Da
• Isoelectric Point: 6.7284

>AT224951 WP_125462792.1 NZ_CP087165:c1122253-1121966 [Pseudomonas sichuanensis]
MSRLFDELMESVVEMDEIIQGKRQPSRQFEIDALQVREIRKATGLSQVRFAEMIDVQVATLRNWEQGRREPTGPAKALLR
AIHNDPDHVLKALSH

Nucleotide

• Length: 288 bp

>AT224951 NZ_CP120638:787512-787760 [Rhizobium sp. 32-5/1]
ATGGCCAGTACGATCCTTGCGGAGACCAGCGCCAGCATCTCCGAACTGAAACGAAATCCGATGGGCACGGTCGCGGCCGG
CGATGGTTTTCCAGTGGCTATTCTCAACCACAACGAGCCGGCCTTTTACTGCGTTCCCGCTCCCGCCTTCGAGGCATTGA
TGAACCGTCTCGAAGATCTGGAACTCAACAGGATTGCCGATTCCCGAATCGGTGAACCGGTCATTCCGGTTGAGATCGAT
GCGCTATAA