Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4752529..4753115 Replicon chromosome
Accession NZ_CP087160
Organism Pseudomonas sp. CIP-10

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag LMH93_RS21435 Protein ID WP_003252277.1
Coordinates 4752529..4752807 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag LMH93_RS21440 Protein ID WP_003252274.1
Coordinates 4752816..4753115 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMH93_RS21425 (LMH93_21425) 4749317..4750513 + 1197 WP_230064572.1 succinyldiaminopimelate transaminase -
LMH93_RS21430 (LMH93_21430) 4750632..4752278 - 1647 WP_060546238.1 Na+/H+ antiporter -
LMH93_RS21435 (LMH93_21435) 4752529..4752807 + 279 WP_003252277.1 type II toxin-antitoxin system toxin GraT Toxin
LMH93_RS21440 (LMH93_21440) 4752816..4753115 + 300 WP_003252274.1 type II toxin-antitoxin system antitoxin GraA Antitoxin
LMH93_RS21445 (LMH93_21445) 4753240..4753587 + 348 WP_003252272.1 ArsC family reductase -
LMH93_RS21450 (LMH93_21450) 4753619..4754653 + 1035 WP_003252270.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
LMH93_RS21455 (LMH93_21455) 4754735..4755940 + 1206 WP_003252268.1 cysteine desulfurase -
LMH93_RS21460 (LMH93_21460) 4755937..4756347 + 411 WP_003252266.1 SufE family protein -
LMH93_RS21465 (LMH93_21465) 4756456..4757265 - 810 WP_230064573.1 tRNA cyclic N6-threonylcarbamoyladenosine(37) synthase TcdA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(8-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-90)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10445.67 Da        Isoelectric Point: 7.2448

>T224946 WP_003252277.1 NZ_CP087160:4752529-4752807 [Pseudomonas sp. CIP-10]
MIQSFSCADTEALFTTGKTRRWSDIKSVAERKLATLDAATELRDLRSPPGNRLESLSGNRAGQHSIRVNDQWRLCFSWTE
HGPVNVEIVDYH

Download         Length: 279 bp

>T224946 NZ_CP120634:c109725-109420 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10891.52 Da        Isoelectric Point: 5.6714

>AT224946 WP_003252274.1 NZ_CP087160:4752816-4753115 [Pseudomonas sp. CIP-10]
MLKNGMRPIHPGEILREDFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA

Download         Length: 300 bp

>AT224946 NZ_CP120634:c109945-109727 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References