Detailed information of TA system TA224943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 1838839..1839568
• Replicon: chromosome
• Accession: NZ_CP087160
• Organism: Pseudomonas sp. CIP-10

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: A0A550F6I7
• Locus tag: LMH93_RS08340
• Protein ID: WP_019750831.1
• Coordinates: 1838839..1839135 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: -
• Locus tag: LMH93_RS08345
• Protein ID: WP_230065005.1
• Coordinates: 1839137..1839568 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMH93_RS08320 (LMH93_08320)	1834475..1836100	+	1626	WP_230065004.1	twin-arginine translocation signal domain-containing protein	-
LMH93_RS08325 (LMH93_08325)	1836222..1836698	+	477	WP_003254241.1	sigma-70 family RNA polymerase sigma factor	-
LMH93_RS08330 (LMH93_08330)	1836867..1837346	+	480	WP_012051716.1	hypothetical protein	-
LMH93_RS08335 (LMH93_08335)	1837419..1838744	+	1326	WP_004573659.1	MFS transporter	-
LMH93_RS08340 (LMH93_08340)	1838839..1839135	+	297	WP_019750831.1	type II toxin-antitoxin system MqsR family toxin	Toxin
LMH93_RS08345 (LMH93_08345)	1839137..1839568	+	432	WP_230065005.1	type II toxin-antitoxin system MqsA family antitoxin	Antitoxin
LMH93_RS08350 (LMH93_08350)	1839606..1841288	-	1683	WP_023389616.1	electron transfer flavoprotein-ubiquinone oxidoreductase	-
LMH93_RS08355 (LMH93_08355)	1841824..1842573	+	750	WP_110745644.1	electron transfer flavoprotein subunit beta/FixA family protein	-
LMH93_RS08360 (LMH93_08360)	1842574..1843503	+	930	WP_003254230.1	FAD-binding protein	-
LMH93_RS08365 (LMH93_08365)	1843579..1844400	+	822	WP_230065006.1	transporter substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (74-109)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11094.88 Da
• Isoelectric Point: 9.8759

>T224943 WP_019750831.1 NZ_CP087160:1838839-1839135 [Pseudomonas sp. CIP-10]
MEKRTPHCPLHRLQVLLSQGKVRTTQAATLGARSLGLGPYDMLDVVRGLSPGDFHKSMTSYNDHRIWQDVYRAQTAVGRV
YLKLTVVDDLLILSFKEL

Nucleotide

• Length: 297 bp

>T224943 NZ_CP120633:c4601717-4601499 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15691.92 Da
• Isoelectric Point: 4.9927

>AT224943 WP_230065005.1 NZ_CP087160:1839137-1839568 [Pseudomonas sp. CIP-10]
MKCPLCGGAELVAQSRDMPYRYKGEATVIPDIFGDYCPTCGEAVLGMDEAQRMSDLMTAFERKVNAAVVDPGFIAAMRRK
FDLDQREAGEIFGGGVNAFSRYENGKTTPPVALVKLLKLLDRHPELFEEVRTDNGSQQKAPGS

Nucleotide

• Length: 432 bp

>AT224943 NZ_CP120633:c4602117-4601743 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A550F6I7