Detailed information of TA system TA224937

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34998..35267
• Replicon: plasmid unnamed2
• Accession: NZ_CP087153
• Organism: Klebsiella pneumoniae strain KP14

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LOX93_RS28370
• Protein ID: WP_001372321.1
• Coordinates: 35142..35267 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	34998..35063 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOX93_RS28340	30708..31235	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
LOX93_RS28345	31293..31526	+	234	WP_000006003.1	DUF905 family protein	-
LOX93_RS28350	31587..33610	+	2024	Protein_43	ParB/RepB/Spo0J family partition protein	-
LOX93_RS28355	33679..34113	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
LOX93_RS28360	34110..34829	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	34841..35065	+	225	NuclAT_0	-	-
-	34841..35065	+	225	NuclAT_0	-	-
-	34841..35065	+	225	NuclAT_0	-	-
-	34841..35065	+	225	NuclAT_0	-	-
-	34998..35063	-	66	-	-	Antitoxin
LOX93_RS28365	35051..35200	+	150	Protein_46	plasmid maintenance protein Mok	-
LOX93_RS28370	35142..35267	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
LOX93_RS28375	35586..35882	-	297	Protein_48	hypothetical protein	-
LOX93_RS28380	36182..36478	+	297	WP_001272251.1	hypothetical protein	-
LOX93_RS28385	36589..37410	+	822	WP_001234445.1	DUF932 domain-containing protein	-
LOX93_RS28390	37707..38354	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
LOX93_RS28395	38631..39014	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LOX93_RS28400	39295..39999	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-41	-	1..133129	133129

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T224937 WP_001372321.1 NZ_CP087153:35142-35267 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T224937 NZ_CP120633:3480126-3480437 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT224937 NZ_CP087153:c35063-34998 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin