Detailed information of TA system TA022493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 123890..124397
• Replicon: chromosome
• Accession: NC_009900
• Organism: Rickettsia massiliae MTU5

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A8F0J4
• Locus tag: RMA_RS00700
• Protein ID: WP_012152410.1
• Coordinates: 123890..124153 (-)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: H6QKN5
• Locus tag: RMA_RS00705
• Protein ID: WP_012152411.1
• Coordinates: 124146..124397 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RMA_RS09135	118974..119145	+	172	Protein_129	palindromic element RPE4 domain-containing protein	-
RMA_RS00675	119391..119772	+	382	Protein_130	hypothetical protein	-
RMA_RS09735	119905..120315	+	411	WP_187145906.1	Sca4 family protein	-
RMA_RS00685	120427..121818	-	1392	WP_041404479.1	lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase	-
RMA_RS00690	121797..122474	-	678	WP_041404480.1	lysophospholipid acyltransferase family protein	-
RMA_RS00695	122625..123830	-	1206	WP_041404481.1	pyridoxal phosphate-dependent aminotransferase	-
RMA_RS00700	123890..124153	-	264	WP_012152410.1	Txe/YoeB family addiction module toxin	Toxin
RMA_RS00705	124146..124397	-	252	WP_012152411.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
RMA_RS00710	124539..125543	+	1005	WP_012152412.1	methyltransferase domain-containing protein	-
RMA_RS00715	125548..126303	+	756	WP_041404936.1	VacJ family lipoprotein	-
RMA_RS00720	126323..126922	+	600	WP_041404482.1	phospholipid-binding protein MlaC	-
RMA_RS00725	127109..128128	+	1020	WP_041404483.1	ribose-phosphate diphosphokinase	-
RMA_RS00730	128125..129192	+	1068	WP_014408186.1	alanine racemase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10626.37 Da
• Isoelectric Point: 9.3100

>T22493 WP_012152410.1 NC_009900:c124153-123890 [Rickettsia massiliae MTU5]
MIEIEWTLEAAEDLAYWKKYDIKKYERIKLLIKNIQEVPFTGIGKPESLKDILSDLWSRRINHEHRLIYSVNTKQIIIYN
CRFHYKN

Nucleotide

• Length: 264 bp

>T22493 NC_009900:c124153-123890 [Rickettsia massiliae MTU5]
ATGATTGAGATTGAGTGGACACTAGAAGCAGCGGAGGATTTAGCGTATTGGAAAAAATACGATATTAAAAAATATGAACG
TATTAAACTTCTAATAAAAAATATCCAAGAAGTACCGTTTACAGGTATAGGTAAGCCCGAATCTTTAAAAGATATATTAT
CAGATTTATGGTCACGTAGAATTAACCACGAACATAGACTAATATATTCTGTCAATACTAAACAAATTATAATATATAAT
TGTAGATTTCATTATAAAAATTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9544.81 Da
• Isoelectric Point: 9.4889

>AT22493 WP_012152411.1 NC_009900:c124397-124146 [Rickettsia massiliae MTU5]
MHSISGTSFRKNLSSVLNTVENDHVPYLIKRKNHKNIILLTEEEYESTKETLYLLSNPANANRIKKSIEQAKRGEFAKVN
LDD

Nucleotide

• Length: 252 bp

>AT22493 NC_009900:c124397-124146 [Rickettsia massiliae MTU5]
ATGCACTCAATTAGCGGCACTTCATTTAGAAAAAACTTAAGCTCTGTACTAAATACCGTAGAAAACGATCATGTCCCTTA
TCTTATTAAAAGAAAGAATCATAAGAATATTATTCTTTTAACCGAAGAAGAATATGAATCTACAAAAGAAACATTATATT
TATTATCTAATCCGGCTAATGCGAATCGAATAAAAAAATCTATCGAACAAGCAAAAAGAGGAGAGTTTGCTAAAGTTAAT
TTAGATGATTGA

Structures

Toxin

• AlphaFold DB: A8F0J4

Antitoxin

• AlphaFold DB: H6QKN5