Detailed information of TA system TA224914

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 132033..132286
• Replicon: plasmid unnamed2
• Accession: NZ_CP087148
• Organism: Klebsiella pneumoniae strain KP16

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LOX92_RS28975
• Protein ID: WP_001312851.1
• Coordinates: 132137..132286 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	132033..132092 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOX92_RS28935 (127415)	127415..127759	-	345	Protein_161	IS6-like element IS26 family transposase	-
LOX92_RS28940 (127811)	127811..128515	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LOX92_RS28945 (128540)	128540..128740	+	201	WP_072354025.1	hypothetical protein	-
LOX92_RS28950 (128760)	128760..129506	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
LOX92_RS28955 (129561)	129561..130121	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LOX92_RS28960 (130253)	130253..130453	+	201	WP_015059022.1	hypothetical protein	-
LOX92_RS28965 (130839)	130839..131438	+	600	WP_032083981.1	PIN domain-containing protein	-
LOX92_RS28970 (131500)	131500..131832	+	333	WP_152916585.1	hypothetical protein	-
- (132033)	132033..132092	-	60	NuclAT_1	-	Antitoxin
- (132033)	132033..132092	-	60	NuclAT_1	-	Antitoxin
- (132033)	132033..132092	-	60	NuclAT_1	-	Antitoxin
- (132033)	132033..132092	-	60	NuclAT_1	-	Antitoxin
LOX92_RS28975 (132137)	132137..132286	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LOX92_RS28980 (132570)	132570..132818	+	249	WP_000083837.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-41	-	1..133129	133129

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T224914 WP_001312851.1 NZ_CP087148:132137-132286 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T224914 NZ_CP120618:c3591456-3590833 [Priestia megaterium]
ATGCAGTTTATTAGTGATATCGAAATTTGGTATGATAAAGACGCTAAACGTTATCGAAATAAAGAGACAGGGCAGTTTAC
TCGTGGTCCAAATCTTGTTCACGAAGCTGGAAGCGGGAACGTTCAACCTTTGGAAGTACCGGTAGTAGAATCAGTTACAG
TTAATAAGCAGAATGATTCAATGAAGGGTACGTCAGTGTTTGATCCTGATTTAGTAAAAGCATATTTAAAGGATGTTGAA
TCCAAAACAAGGTTAAAGGTTCACAAAATACAGGTGGAATTTCTAAAAGAATCCTTAAGAAATAATAAATTTGAAAAATT
AACACCTAAAGAAACAGCTAAGCATAGAGGTAGATTTACTACAAGCCTAAAAAACAAGTTAATTGAAGAATGGGAAGAAA
AAACCAATCAAAAGTGGCCACGATATACAGAGGAAGTATATGATCAAAGTGGAGAAATTGCTAGACATATTGGTCAACCA
TACGATGCTCATCATCTTATAGAAAACAATTTTTCTGGTCCTCATGAATGGTGGAATATTCATCCTGCTAAATACCCTGA
CGAGCATCAGCAGGGGATTCATGGAAAAGGAGCACCATCAAGAATGTTATTCCCTAGGAGGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT224914 NZ_CP087148:c132092-132033 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin