Detailed information of TA system TA224908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 40317..41044
• Replicon: plasmid unnamed1
• Accession: NZ_CP087147
• Organism: Klebsiella pneumoniae strain KP16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LOX92_RS27250
• Protein ID: WP_011251285.1
• Coordinates: 40733..41044 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOX92_RS27245
• Protein ID: WP_011251286.1
• Coordinates: 40317..40736 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOX92_RS27215 (LOX92_27215)	35485..35955	-	471	WP_048333570.1	hypothetical protein	-
LOX92_RS27220 (LOX92_27220)	36268..36903	-	636	WP_223171879.1	hypothetical protein	-
LOX92_RS27225 (LOX92_27225)	37322..37942	+	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
LOX92_RS27230 (LOX92_27230)	37963..38751	+	789	WP_040217257.1	hypothetical protein	-
LOX92_RS27235 (LOX92_27235)	38765..39130	+	366	WP_048333448.1	hypothetical protein	-
LOX92_RS27240 (LOX92_27240)	39202..40170	-	969	WP_074428168.1	IS5 family transposase	-
LOX92_RS27245 (LOX92_27245)	40317..40736	-	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LOX92_RS27250 (LOX92_27250)	40733..41044	-	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LOX92_RS27255 (LOX92_27255)	41249..41686	-	438	Protein_46	DDE-type integrase/transposase/recombinase	-
LOX92_RS27260 (LOX92_27260)	41821..42518	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
LOX92_RS27265 (LOX92_27265)	43904..44554	-	651	WP_068893702.1	DUF1173 family protein	-
LOX92_RS27270 (LOX92_27270)	44587..44853	-	267	WP_223175074.1	DUF1173 family protein	-
LOX92_RS27275 (LOX92_27275)	45031..45525	+	495	WP_004212794.1	thermonuclease family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2	1..197415	197415
	-	inside	IScluster/Tn	-	-	22688..47711	25023

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T224908 WP_011251285.1 NZ_CP087147:c41044-40733 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T224908 NZ_CP120613:2936340-2936516 [Bacillus subtilis]
GTGCTTGAGAAAATGGGTATCGTAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGGTAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT224908 WP_011251286.1 NZ_CP087147:c40736-40317 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT224908 NZ_CP120613:c2936399-2936299 [Bacillus subtilis]
GATTGTAAGAACCGTTAAAGATATGAGGAAAGCAACTACGATACCCATTTTCTCAAGCACTATGTACACCTCCTTTCCTA
TGACTCTATTATAACATAATT

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1