Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 40317..41044 Replicon plasmid unnamed1
Accession NZ_CP087143
Organism Klebsiella pneumoniae strain KP15

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3D9W1
Locus tag LOX91_RS27270 Protein ID WP_011251285.1
Coordinates 40733..41044 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LOX91_RS27265 Protein ID WP_011251286.1
Coordinates 40317..40736 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX91_RS27235 (LOX91_27235) 35485..35955 - 471 WP_048333570.1 hypothetical protein -
LOX91_RS27240 (LOX91_27240) 36268..36903 - 636 WP_223171879.1 hypothetical protein -
LOX91_RS27245 (LOX91_27245) 37322..37942 + 621 WP_223175004.1 conjugative transfer protein MobI(A/C) -
LOX91_RS27250 (LOX91_27250) 37963..38751 + 789 WP_040217257.1 hypothetical protein -
LOX91_RS27255 (LOX91_27255) 38765..39130 + 366 WP_048333448.1 hypothetical protein -
LOX91_RS27260 (LOX91_27260) 39202..40170 - 969 WP_074428168.1 IS5 family transposase -
LOX91_RS27265 (LOX91_27265) 40317..40736 - 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
LOX91_RS27270 (LOX91_27270) 40733..41044 - 312 WP_011251285.1 type II toxin-antitoxin system HigB family toxin Toxin
LOX91_RS27275 (LOX91_27275) 41249..41686 - 438 Protein_46 DDE-type integrase/transposase/recombinase -
LOX91_RS27280 (LOX91_27280) 41821..42518 + 698 WP_223175001.1 IS1-like element IS1A family transposase -
LOX91_RS27285 (LOX91_27285) 43904..44554 - 651 WP_068893702.1 DUF1173 family protein -
LOX91_RS27290 (LOX91_27290) 44587..44853 - 267 WP_223175074.1 DUF1173 family protein -
LOX91_RS27295 (LOX91_27295) 45031..45525 + 495 WP_004212794.1 thermonuclease family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..197415 197415
- inside IScluster/Tn - - 22688..47711 25023


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12346.12 Da        Isoelectric Point: 9.7248

>T224881 WP_011251285.1 NZ_CP087143:c41044-40733 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T224881 NZ_CP120568:2757676-2757783 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT224881 WP_011251286.1 NZ_CP087143:c40736-40317 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT224881 NZ_CP120568:c2757626-2757563 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3D9W1

Antitoxin

Source ID Structure

References