Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 349765..350411 Replicon chromosome
Accession NZ_CP087142
Organism Klebsiella pneumoniae strain KP15

Toxin (Protein)


Gene name higB Uniprot ID W8UNE1
Locus tag LOX91_RS01615 Protein ID WP_002920560.1
Coordinates 349765..350112 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag LOX91_RS01620 Protein ID WP_002920557.1
Coordinates 350112..350411 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX91_RS01605 (LOX91_01605) 345691..347124 + 1434 WP_002920564.1 glycogen synthase GlgA -
LOX91_RS01610 (LOX91_01610) 347142..349589 + 2448 WP_002920561.1 glycogen phosphorylase -
LOX91_RS01615 (LOX91_01615) 349765..350112 + 348 WP_002920560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LOX91_RS01620 (LOX91_01620) 350112..350411 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
LOX91_RS01625 (LOX91_01625) 350474..351982 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
LOX91_RS01630 (LOX91_01630) 352187..352516 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
LOX91_RS01635 (LOX91_01635) 352567..353397 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
LOX91_RS01640 (LOX91_01640) 353447..354205 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13502.56 Da        Isoelectric Point: 6.2300

>T224863 WP_002920560.1 NZ_CP087142:349765-350112 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRACFAFDPLRKAI
VLCVGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T224863 NZ_CP120567:c3911639-3911337 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATTGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT224863 WP_002920557.1 NZ_CP087142:350112-350411 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT224863 NZ_CP120567:c3911301-3910960 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CBY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References