Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2083239..2083741 Replicon chromosome
Accession NZ_CP087136
Organism Escherichia coli strain JW5503

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag LOX89_RS10410 Protein ID WP_000767829.1
Coordinates 2083239..2083493 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag LOX89_RS10415 Protein ID WP_001259255.1
Coordinates 2083490..2083741 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX89_RS10380 (2078254) 2078254..2078481 - 228 WP_000234896.1 sulfurtransferase TusA -
LOX89_RS10385 (2078495) 2078495..2079553 - 1059 WP_000492339.1 thiosulfate utilization transporter TsuA/YeeE -
LOX89_RS10390 (2079732) 2079732..2081090 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
LOX89_RS10395 (2081080) 2081080..2081142 - 63 WP_010723108.1 membrane protein YoeI -
LOX89_RS10400 (2081357) 2081357..2082286 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
LOX89_RS10405 (2082332) 2082332..2083156 - 825 WP_000754737.1 SDR family oxidoreductase -
LOX89_RS10410 (2083239) 2083239..2083493 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
LOX89_RS10415 (2083490) 2083490..2083741 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
LOX89_RS10420 (2084024) 2084024..2084074 + 51 WP_001364200.1 his operon leader peptide -
LOX89_RS10425 (2084220) 2084220..2085119 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
LOX89_RS10430 (2085125) 2085125..2086429 + 1305 WP_000009594.1 histidinol dehydrogenase -
LOX89_RS10435 (2086426) 2086426..2087496 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
LOX89_RS10440 (2087496) 2087496..2088563 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T224841 WP_000767829.1 NZ_CP087136:c2083493-2083239 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T224841 NZ_CP120563:4214944-4215285 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT224841 WP_001259255.1 NZ_CP087136:c2083741-2083490 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT224841 NZ_CP120563:c4214949-4214873 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References