224841

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2083239..2083741 Replicon chromosome
Accession NZ_CP087136
Organism Escherichia coli strain JW5503

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag LOX89_RS10410 Protein ID WP_000767829.1
Coordinates 2083239..2083493 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag LOX89_RS10415 Protein ID WP_001259255.1
Coordinates 2083490..2083741 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX89_RS10380 (2078254) 2078254..2078481 - 228 WP_000234896.1 sulfurtransferase TusA -
LOX89_RS10385 (2078495) 2078495..2079553 - 1059 WP_000492339.1 thiosulfate utilization transporter TsuA/YeeE -
LOX89_RS10390 (2079732) 2079732..2081090 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
LOX89_RS10395 (2081080) 2081080..2081142 - 63 WP_010723108.1 membrane protein YoeI -
LOX89_RS10400 (2081357) 2081357..2082286 - 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
LOX89_RS10405 (2082332) 2082332..2083156 - 825 WP_000754737.1 SDR family oxidoreductase -
LOX89_RS10410 (2083239) 2083239..2083493 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
LOX89_RS10415 (2083490) 2083490..2083741 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
LOX89_RS10420 (2084024) 2084024..2084074 + 51 WP_001364200.1 his operon leader peptide -
LOX89_RS10425 (2084220) 2084220..2085119 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
LOX89_RS10430 (2085125) 2085125..2086429 + 1305 WP_000009594.1 histidinol dehydrogenase -
LOX89_RS10435 (2086426) 2086426..2087496 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
LOX89_RS10440 (2087496) 2087496..2088563 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T224841 WP_000767829.1 NZ_CP087136:c2083493-2083239 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T224841 NZ_CP120563:4214944-4215285 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT224841 WP_001259255.1 NZ_CP087136:c2083741-2083490 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT224841 NZ_CP120563:c4214949-4214873 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References