Detailed information of TA system TA224810

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 5570379..5571106
• Replicon: chromosome
• Accession: NZ_CP087123
• Organism: Klebsiella pneumoniae strain TH12908

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: LOX65_RS26910
• Protein ID: WP_011251285.1
• Coordinates: 5570379..5570690 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: LOX65_RS26915
• Protein ID: WP_011251286.1
• Coordinates: 5570687..5571106 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOX65_RS26870 (5565440)	5565440..5565955	-	516	WP_041937820.1	hypothetical protein	-
LOX65_RS26875 (5566063)	5566063..5566638	-	576	WP_074424927.1	hypothetical protein	-
LOX65_RS26880 (5566755)	5566755..5567711	-	957	WP_011251280.1	DsbA family protein	-
LOX65_RS26885 (5567771)	5567771..5568112	-	342	WP_011251281.1	hypothetical protein	-
LOX65_RS26890 (5568126)	5568126..5568437	-	312	WP_011251282.1	hypothetical protein	-
LOX65_RS26895 (5568454)	5568454..5568903	-	450	WP_011251283.1	thioredoxin fold domain-containing protein	-
LOX65_RS26905 (5569737)	5569737..5570174	+	438	Protein_5263	DDE-type integrase/transposase/recombinase	-
LOX65_RS26910 (5570379)	5570379..5570690	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
LOX65_RS26915 (5570687)	5570687..5571106	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
LOX65_RS26920 (5571253)	5571253..5572221	+	969	WP_074428168.1	IS5 family transposase	-
LOX65_RS26925 (5572293)	5572293..5572658	-	366	WP_048333448.1	hypothetical protein	-
LOX65_RS26930 (5572672)	5572672..5573460	-	789	WP_040217257.1	hypothetical protein	-
LOX65_RS26935 (5573481)	5573481..5574101	-	621	WP_223175004.1	conjugative transfer protein MobI(A/C)	-
LOX65_RS26940 (5574520)	5574520..5575155	+	636	WP_223171879.1	hypothetical protein	-
LOX65_RS26945 (5575468)	5575468..5575938	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	5414866..5612194	197328
	-	inside	IScluster/Tn	-	-	5568905..5581227	12322

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T224810 WP_011251285.1 NZ_CP087123:5570379-5570690 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T224810 NZ_CP120557:c2233460-2233125 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT224810 WP_011251286.1 NZ_CP087123:5570687-5571106 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT224810 NZ_CP120557:c2233708-2233460 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1