Detailed information of TA system TA022480

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53100..53354
• Replicon: plasmid pAPEC-O1-ColBM
• Accession: NC_009837
• Organism: Escherichia coli APEC O1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: APECO1_RS26005
• Protein ID: WP_001351576.1
• Coordinates: 53148..53354 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	53100..53161 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
APECO1_RS25985	49848..50594	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
APECO1_RS25990	50653..51513	+	861	WP_000704513.1	alpha/beta hydrolase	-
APECO1_RS25995	51616..52176	+	561	WP_000139315.1	fertility inhibition protein FinO	-
APECO1_RS26000	52312..52524	+	213	WP_001299730.1	ANR family transcriptional regulator	-
APECO1_RS31665	52770..52844	+	75	Protein_61	endonuclease	-
-	53100..53161	-	62	NuclAT_1	-	Antitoxin
-	53100..53161	-	62	NuclAT_1	-	Antitoxin
-	53100..53161	-	62	NuclAT_1	-	Antitoxin
-	53100..53161	-	62	NuclAT_1	-	Antitoxin
APECO1_RS26005	53148..53354	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
APECO1_RS26010	53638..53895	+	258	WP_000083845.1	replication regulatory protein RepA	-
APECO1_RS26015	54127..54201	+	75	WP_001442103.1	RepA leader peptide Tap	-
APECO1_RS26020	54194..54676	+	483	WP_001273588.1	hypothetical protein	-
APECO1_RS26025	54669..55526	+	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
APECO1_RS26030	56489..56740	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
APECO1_RS26035	56737..57024	+	288	WP_000222767.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	vat / iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..174241	174241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T22480 WP_001351576.1 NC_009837:53148-53354 [Escherichia coli APEC O1]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T22480 NC_009837:53148-53354 [Escherichia coli APEC O1]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT22480 NC_009837:c53161-53100 [Escherichia coli APEC O1]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin