Detailed information of TA system TA224785

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 5489777..5490030
• Replicon: chromosome
• Accession: NZ_CP087122
• Organism: Klebsiella pneumoniae strain TH12852

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: LOX64_RS26760
• Protein ID: WP_001312851.1
• Coordinates: 5489881..5490030 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	5489777..5489836 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOX64_RS26735 (5486504)	5486504..5487250	+	747	WP_229982420.1	conjugal transfer pilus acetylase TraX	-
LOX64_RS26740 (5487305)	5487305..5487865	+	561	WP_000139328.1	fertility inhibition protein FinO	-
LOX64_RS26745 (5487997)	5487997..5488197	+	201	WP_015059022.1	hypothetical protein	-
LOX64_RS26750 (5488583)	5488583..5489182	+	600	WP_032083981.1	PIN domain-containing protein	-
LOX64_RS26755 (5489244)	5489244..5489576	+	333	WP_152916585.1	hypothetical protein	-
- (5489777)	5489777..5489836	-	60	NuclAT_1	-	Antitoxin
- (5489777)	5489777..5489836	-	60	NuclAT_1	-	Antitoxin
- (5489777)	5489777..5489836	-	60	NuclAT_1	-	Antitoxin
- (5489777)	5489777..5489836	-	60	NuclAT_1	-	Antitoxin
LOX64_RS26760 (5489881)	5489881..5490030	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
LOX64_RS26765 (5490314)	5490314..5490562	+	249	WP_000083837.1	replication regulatory protein RepA	-
LOX64_RS26770 (5490677)	5490677..5490855	+	179	Protein_5228	protein CopA/IncA	-
LOX64_RS26780 (5490874)	5490874..5491731	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
LOX64_RS26785 (5492670)	5492670..5493323	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
LOX64_RS26790 (5493416)	5493416..5493673	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
LOX64_RS26795 (5493606)	5493606..5494007	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
LOX64_RS26800 (5494256)	5494256..5494671	+	416	Protein_5233	IS1-like element IS1B family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	fosA3 / blaTEM-1B / blaCTX-M-55	-	5473944..5504636	30692

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T224785 WP_001312851.1 NZ_CP087122:5489881-5490030 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T224785 NZ_CP120549:2619635-2619904 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACACGATT
ACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTACATCCGTTAA
GCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAATGGCGATGCA
TACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Nucleotide

• Length: 60 bp

>AT224785 NZ_CP087122:c5489836-5489777 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin