Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 5334344..5335018 Replicon chromosome
Accession NZ_CP087122
Organism Klebsiella pneumoniae strain TH12852

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag LOX64_RS25860 Protein ID WP_012539985.1
Coordinates 5334695..5335018 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LOX64_RS25855 Protein ID WP_065810615.1
Coordinates 5334344..5334646 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX64_RS25825 (5330018) 5330018..5330851 - 834 WP_004136823.1 oxidoreductase -
LOX64_RS25830 (5330956) 5330956..5331876 + 921 WP_012540028.1 AraC family transcriptional regulator -
LOX64_RS25835 (5332439) 5332439..5332591 + 153 Protein_5042 transposase -
LOX64_RS25840 (5332865) 5332865..5333161 + 297 WP_224275728.1 TonB family protein -
LOX64_RS25845 (5333186) 5333186..5333728 + 543 WP_012539969.1 GNAT family N-acetyltransferase -
LOX64_RS25850 (5334017) 5334017..5334301 + 285 WP_065810614.1 hypothetical protein -
LOX64_RS25855 (5334344) 5334344..5334646 - 303 WP_065810615.1 NadS family protein Antitoxin
LOX64_RS25860 (5334695) 5334695..5335018 - 324 WP_012539985.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LOX64_RS25865 (5335312) 5335312..5335590 + 279 WP_224275725.1 transposase -
LOX64_RS25870 (5335931) 5335931..5336566 - 636 WP_012540037.1 DUF2268 domain-containing putative Zn-dependent protease -
LOX64_RS25875 (5337947) 5337947..5338312 - 366 WP_040107964.1 DUF596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(45-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-83)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 12358.27 Da        Isoelectric Point: 9.6240

>T224782 WP_012539985.1 NZ_CP087122:c5335018-5334695 [Klebsiella pneumoniae]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDDLSEKEKAVMKALTQQLK

Download         Length: 324 bp

>T224782 NZ_CP120549:c1959942-1959568 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11256.15 Da        Isoelectric Point: 6.8718

>AT224782 WP_065810615.1 NZ_CP087122:c5334646-5334344 [Klebsiella pneumoniae]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRIPSGIALK
MLCLIEREPTLIDTLRGIHV

Download         Length: 303 bp

>AT224782 NZ_CP120549:c1960399-1960031 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
CCAGCGATGCAGATGCGTACCCCCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCAATCAGCATACCGTCACGCTGTATGCGAAGGGGCTGGCCTGTGAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References