Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3788680..3789277 Replicon chromosome
Accession NZ_CP087122
Organism Klebsiella pneumoniae strain TH12852

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag LOX64_RS18535 Protein ID WP_004142563.1
Coordinates 3788960..3789277 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag LOX64_RS18530 Protein ID WP_004142561.1
Coordinates 3788680..3788967 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOX64_RS18500 (3784760) 3784760..3785008 + 249 WP_002893037.1 DUF1158 domain-containing protein -
LOX64_RS18505 (3785026) 3785026..3785367 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
LOX64_RS18510 (3785398) 3785398..3786513 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
LOX64_RS18515 (3786693) 3786693..3787274 + 582 WP_004176968.1 TetR/AcrR family transcriptional regulator -
LOX64_RS18520 (3787274) 3787274..3787642 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
LOX64_RS18525 (3787762) 3787762..3788415 + 654 WP_004176973.1 oxygen-insensitive NAD(P)H nitroreductase -
LOX64_RS18530 (3788680) 3788680..3788967 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
LOX64_RS18535 (3788960) 3788960..3789277 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LOX64_RS18540 (3789462) 3789462..3790505 - 1044 WP_040236579.1 DUF2157 domain-containing protein -
LOX64_RS18545 (3791175) 3791175..3792041 - 867 WP_064164470.1 helix-turn-helix transcriptional regulator -
LOX64_RS18550 (3792150) 3792150..3793577 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T224776 WP_004142563.1 NZ_CP087122:c3789277-3788960 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T224776 NZ_CP120549:c602438-601974 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT224776 WP_004142561.1 NZ_CP087122:c3788967-3788680 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT224776 NZ_CP120549:c602773-602438 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References