Detailed information of TA system TA224762

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1341098..1341725
• Replicon: chromosome
• Accession: NZ_CP087114
• Organism: Bartonella machadoae strain 46A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: LNM86_RS06520
• Protein ID: WP_241438842.1
• Coordinates: 1341098..1341412 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: LNM86_RS06525
• Protein ID: WP_004861504.1
• Coordinates: 1341429..1341725 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LNM86_RS06500 (LNM86_06500)	1336771..1338312	-	1542	WP_241438185.1	phage portal protein	-
LNM86_RS06505 (LNM86_06505)	1338312..1338560	-	249	WP_241438023.1	hypothetical protein	-
LNM86_RS06510 (LNM86_06510)	1338564..1340492	-	1929	WP_241438186.1	phage terminase large subunit family protein	-
LNM86_RS06515 (LNM86_06515)	1340485..1341045	-	561	WP_241438841.1	hypothetical protein	-
LNM86_RS06520 (LNM86_06520)	1341098..1341412	+	315	WP_241438842.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LNM86_RS06525 (LNM86_06525)	1341429..1341725	+	297	WP_004861504.1	HigA family addiction module antitoxin	Antitoxin
LNM86_RS06530 (LNM86_06530)	1341770..1342048	-	279	WP_241438844.1	hypothetical protein	-
LNM86_RS06535 (LNM86_06535)	1342045..1342779	-	735	WP_241438846.1	antA/AntB antirepressor family protein	-
LNM86_RS06540 (LNM86_06540)	1343340..1343921	-	582	WP_241438848.1	hypothetical protein	-
LNM86_RS06545 (LNM86_06545)	1343925..1344245	-	321	WP_241438941.1	hypothetical protein	-
LNM86_RS06550 (LNM86_06550)	1344566..1344961	-	396	WP_241438849.1	type II toxin-antitoxin system HicB family antitoxin	-
LNM86_RS06555 (LNM86_06555)	1344958..1345158	-	201	WP_241438850.1	type II toxin-antitoxin system HicA family toxin	-
LNM86_RS06560 (LNM86_06560)	1345228..1345764	-	537	WP_241438851.1	hypothetical protein	-
LNM86_RS06565 (LNM86_06565)	1345774..1346226	-	453	WP_241438852.1	single-stranded DNA-binding protein	-
LNM86_RS06570 (LNM86_06570)	1346419..1346718	+	300	WP_241438853.1	BrnT family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1209361..1408772	199411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (12-104)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-93)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12155.92 Da
• Isoelectric Point: 9.0985

>T224762 WP_241438842.1 NZ_CP087114:1341098-1341412 [Bartonella machadoae]
MIHKRTNEGDFVIESFADKRCKDLLEGNPPRGFPSTLVRIAQRKLFMLDKAVDLKDLRSPPGNRLEALKGERKGQYSIRI
NDQFRICFEWRSNGAYEVEIVDYH

Nucleotide

• Length: 315 bp

>T224762 NZ_CP120508:c2603412-2603146 [Paraburkholderia sp. SUR17]
ATGAGCGGCGCTTTGAACATCATGTGGACTGCCGAGGCCTGGGACGATTACGTCTACTGGCAGGGGCAGGACAAAAGGAC
GTTGAAGCGCATCAATCAACTCATCAAGGATATGCAGCGCTCCCCATTCGAAGGCATCGGCAAACCCGAGTCGCTGAAGG
AAAACCTGACGGGTTTTTGGTCGCGCCGCATTGATGAAACGAATCGCTTGGTCTACGAAGTCGCGAGTACGCAAATCAAT
ATCGTTTCGTGCCGGTACCACTACTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11320.99 Da
• Isoelectric Point: 5.1546

>AT224762 WP_004861504.1 NZ_CP087114:1341429-1341725 [Bartonella machadoae]
MKNYIAIHPGEILREEYLKEYALSAYALAKALNVPRTRIERIVAENSPVTPDTALRLAYFFDTTAEFWLNMQAAYDVSIL
QTEKADEFAKINKFECRV

Nucleotide

• Length: 297 bp

>AT224762 NZ_CP120508:c2603669-2603409 [Paraburkholderia sp. SUR17]
ATGCGCACCATCCCATTCACCGACGCCCGCGCCAATTTGAAGCGCGTCATTGACCAGGTTGTTGATGATGTGGACGTAAC
GCTCATTACCCGCCGCGACGCACCCAATGCGGTTGTCATGTCCCAGGAACATTACGATAGCTTGATGGAAACGGTCCATC
TGCTGCGCTCCCCGGCAAACGTAGCTCATCTGGAGCGCTCTATCGCGCAGTCACGCGCGGGCAAGGTCAAGCCCCGCAAG
TTGGTCGACGACAGCGAATGA