Detailed information of TA system TA224746

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 3845203..3845615
• Replicon: chromosome
• Accession: NZ_CP087110
• Organism: Escherichia coli strain PJ-T13

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: LM520_RS18875
• Protein ID: WP_000132601.1
• Coordinates: 3845274..3845615 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	3845203..3845279 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM520_RS18865 (3842066)	3842066..3843655	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
LM520_RS18870 (3843652)	3843652..3845046	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
- (3845203)	3845203..3845279	-	77	NuclAT_14	-	Antitoxin
- (3845203)	3845203..3845279	-	77	NuclAT_14	-	Antitoxin
- (3845203)	3845203..3845279	-	77	NuclAT_14	-	Antitoxin
- (3845203)	3845203..3845279	-	77	NuclAT_14	-	Antitoxin
- (3845203)	3845203..3845279	-	77	NuclAT_15	-	Antitoxin
- (3845203)	3845203..3845279	-	77	NuclAT_15	-	Antitoxin
- (3845203)	3845203..3845279	-	77	NuclAT_15	-	Antitoxin
- (3845203)	3845203..3845279	-	77	NuclAT_15	-	Antitoxin
LM520_RS18875 (3845274)	3845274..3845615	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
LM520_RS18880 (3845777)	3845777..3847156	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
LM520_RS18885 (3847156)	3847156..3848202	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T224746 WP_000132601.1 NZ_CP087110:3845274-3845615 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T224746 NZ_CP120423:c12234-11872 [Staphylococcus epidermidis]
ATGATTAGAAGAGGAGATGTTTATTTAGCGGATTTATCACCAGTTCAAGGGTCTGAACAAGGGGGAGTAAGACCTGTAGT
TATCATTCAAAATGATACTGGTAATAAATATAGTCCAACTGTAATTGTAGCTGCGATTACTGGTAGGATTAATAAAGCGA
AAATACCAACCCACGTAGAAATTGAAAAGAAAAAGTATAAATTAGACAAAGATTCAGTTATTCTTCTTGAACAAATTAGA
ACACTAGATAAAAAGCGTTTAAAAGAAAAATTAACATTTTTATCAGAGAGTAAAATGATAGAGGTTGATAATGCCTTAGA
TATTAGTTTGGGATTAAATAACTTTGATCATCATAAATCTTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT224746 NZ_CP087110:c3845279-3845203 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin