Detailed information of TA system TA224725

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 572195..572888
• Replicon: chromosome
• Accession: NZ_CP087110
• Organism: Escherichia coli strain PJ-T13

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: LM520_RS02865
• Protein ID: WP_000415584.1
• Coordinates: 572195..572491 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: LM520_RS02870
• Protein ID: WP_000650107.1
• Coordinates: 572493..572888 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM520_RS02830 (567283)	567283..567597	-	315	WP_000958598.1	putative quinol monooxygenase	-
LM520_RS02835 (567628)	567628..568209	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
LM520_RS02840 (568528)	568528..568860	+	333	WP_000917684.1	DUF2645 family protein	-
LM520_RS02845 (568906)	568906..570255	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
LM520_RS02850 (570252)	570252..570911	-	660	WP_001221495.1	quorum sensing response regulator transcription factor QseB	-
LM520_RS02855 (571063)	571063..571455	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
LM520_RS02860 (571508)	571508..571990	+	483	WP_000183505.1	GyrI-like domain-containing protein	-
LM520_RS02865 (572195)	572195..572491	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
LM520_RS02870 (572493)	572493..572888	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
LM520_RS02875 (573021)	573021..574628	+	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
LM520_RS02880 (574766)	574766..577024	+	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T224725 WP_000415584.1 NZ_CP087110:572195-572491 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T224725 NZ_CP120398:c1404432-1404214 [Salmonella enterica subsp. enterica serovar Typhi]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT224725 WP_000650107.1 NZ_CP087110:572493-572888 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT224725 NZ_CP120398:c1404834-1404460 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65