Detailed information of TA system TA224703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 6744..7258
• Replicon: plasmid P9
• Accession: NZ_CP087081
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC36-14

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE32_RS24680
• Protein ID: WP_224557656.1
• Coordinates: 6992..7258 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A074T9H5
• Locus tag: LOE32_RS24675
• Protein ID: WP_036743750.1
• Coordinates: 6744..6995 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE32_RS24655 (LOE32_24645)	2266..3123	-	858	WP_224557653.1	branched-chain amino acid ABC transporter permease	-
LOE32_RS24660 (LOE32_24650)	3196..4377	-	1182	WP_229940888.1	ABC transporter substrate-binding protein	-
LOE32_RS24665 (LOE32_24655)	4715..5779	+	1065	WP_224557654.1	AraC family transcriptional regulator	-
LOE32_RS24670 (LOE32_24660)	5951..6637	+	687	WP_224557655.1	helix-turn-helix transcriptional regulator	-
LOE32_RS24675 (LOE32_24665)	6744..6995	+	252	WP_036743750.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE32_RS24680 (LOE32_24670)	6992..7258	+	267	WP_224557656.1	Txe/YoeB family addiction module toxin	Toxin
LOE32_RS24685 (LOE32_24675)	7501..8544	-	1044	WP_224557657.1	IS5 family transposase	-
LOE32_RS24690 (LOE32_24680)	8578..8769	-	192	WP_224557658.1	hypothetical protein	-
LOE32_RS24695 (LOE32_24685)	9412..10080	-	669	WP_151654624.1	ParA family protein	-
LOE32_RS24700 (LOE32_24690)	10195..11229	-	1035	WP_151654626.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37719	37719
	-	flank	IS/Tn	-	-	7501..8544	1043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10381.70 Da
• Isoelectric Point: 8.9104

>T224703 WP_224557656.1 NZ_CP087081:6992-7258 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVSTNDKVRDRINELIKQCKRTPFKGSGLPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224703 NZ_CP120396:c3863305-3863018 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGTAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9181.38 Da
• Isoelectric Point: 5.6864

>AT224703 WP_036743750.1 NZ_CP087081:6744-6995 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLDTWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEERE
LTE

Nucleotide

• Length: 252 bp

>AT224703 NZ_CP120396:c3863031-3862729 [Salmonella enterica subsp. enterica serovar Typhi]
ATGAGCATGGTTAAACATAAACGCGGCAACGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGACAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGACGTTATGGAATGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTGAATGCCATTCTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAG

Structures

Antitoxin

• AlphaFold DB: A0A074T9H5