Detailed information of TA system TA224668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 18814..19328
• Replicon: plasmid P8
• Accession: NZ_CP087040
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC52-67

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE43_RS24240
• Protein ID: WP_224557656.1
• Coordinates: 19062..19328 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A074T9H5
• Locus tag: LOE43_RS24235
• Protein ID: WP_036743750.1
• Coordinates: 18814..19065 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE43_RS24215 (LOE43_24205)	14336..15193	-	858	WP_224557653.1	branched-chain amino acid ABC transporter permease	-
LOE43_RS24220 (LOE43_24210)	15266..16447	-	1182	WP_229940888.1	ABC transporter substrate-binding protein	-
LOE43_RS24225 (LOE43_24215)	16785..17849	+	1065	WP_224557654.1	AraC family transcriptional regulator	-
LOE43_RS24230 (LOE43_24220)	18021..18707	+	687	WP_224557655.1	helix-turn-helix transcriptional regulator	-
LOE43_RS24235 (LOE43_24225)	18814..19065	+	252	WP_036743750.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE43_RS24240 (LOE43_24230)	19062..19328	+	267	WP_224557656.1	Txe/YoeB family addiction module toxin	Toxin
LOE43_RS24245 (LOE43_24235)	19571..20614	-	1044	WP_224557657.1	IS5 family transposase	-
LOE43_RS24250 (LOE43_24240)	20648..20839	-	192	WP_224557658.1	hypothetical protein	-
LOE43_RS24255 (LOE43_24245)	21482..22150	-	669	WP_151654624.1	ParA family protein	-
LOE43_RS24260 (LOE43_24250)	22265..23299	-	1035	WP_151654626.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..41440	41440
	-	flank	IS/Tn	-	-	19571..20614	1043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10381.70 Da
• Isoelectric Point: 8.9104

>T224668 WP_224557656.1 NZ_CP087040:19062-19328 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVSTNDKVRDRINELIKQCKRTPFKGSGLPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224668 NZ_CP120393:c4246330-4245839 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGAAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGTGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9181.38 Da
• Isoelectric Point: 5.6864

>AT224668 WP_036743750.1 NZ_CP087040:18814-19065 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLDTWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEERE
LTE

Nucleotide

• Length: 252 bp

>AT224668 NZ_CP120393:c4246620-4246327 [Salmonella enterica subsp. enterica serovar Typhi]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGTGAAACTCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATTGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGTTGGAAGCTGCCCGTGCTGCCGCAGAAGAAGTGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAATTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A074T9H5