Detailed information of TA system TA224657

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-RelB
• Location: 13247..13761
• Replicon: plasmid P5
• Accession: NZ_CP087021
• Organism: Pseudosulfitobacter pseudonitzschiae strain MC52-70

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: LOE45_RS21320
• Protein ID: WP_224557656.1
• Coordinates: 13495..13761 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: A0A074T9H5
• Locus tag: LOE45_RS21315
• Protein ID: WP_036743750.1
• Coordinates: 13247..13498 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOE45_RS21295 (LOE45_21310)	8769..9626	-	858	WP_224557653.1	branched-chain amino acid ABC transporter permease	-
LOE45_RS21300 (LOE45_21315)	9699..10880	-	1182	WP_229940888.1	ABC transporter substrate-binding protein	-
LOE45_RS21305 (LOE45_21320)	11218..12282	+	1065	WP_224557654.1	AraC family transcriptional regulator	-
LOE45_RS21310 (LOE45_21325)	12454..13140	+	687	WP_224557655.1	helix-turn-helix transcriptional regulator	-
LOE45_RS21315 (LOE45_21330)	13247..13498	+	252	WP_036743750.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
LOE45_RS21320 (LOE45_21335)	13495..13761	+	267	WP_224557656.1	Txe/YoeB family addiction module toxin	Toxin
LOE45_RS21325 (LOE45_21340)	14004..15047	-	1044	WP_224557657.1	IS5 family transposase	-
LOE45_RS21330 (LOE45_21345)	15081..15272	-	192	WP_224557658.1	hypothetical protein	-
LOE45_RS21335 (LOE45_21350)	15915..16583	-	669	WP_151654624.1	ParA family protein	-
LOE45_RS21340 (LOE45_21355)	16698..17732	-	1035	WP_151654626.1	replication initiator protein A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..37996	37996
	-	flank	IS/Tn	-	-	14004..15047	1043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10381.70 Da
• Isoelectric Point: 8.9104

>T224657 WP_224557656.1 NZ_CP087021:13495-13761 [Pseudosulfitobacter pseudonitzschiae]
VKLVFSDQAWEDYQYWVSTNDKVRDRINELIKQCKRTPFKGSGLPEPLKGDLTGWWSRRISQEDRMVYRVSGTGDGQSLE
IAQLRFHY

Nucleotide

• Length: 267 bp

>T224657 NZ_CP120392:c4678620-4678246 [Salmonella enterica subsp. enterica serovar Typhi]
ATGGTCAAAGGGTCTGCGCTTTTTGATACCAATATCCTCATCGACCTGTTTAGCGGTCGAATTGAAGCGAAACATGCGCT
GGAAGCGTATCCGCCACAAAATGCGATTAGCCTTATCACATGGATGGAAGTGATGGTGGGTGCTAAGAAATATCATCAGG
AAAACCGCACGCGCATTGCATTAAGTGCGTTCAATATTATTGGCGTAACACAGGAAATCGCGGAACGAAGCGTCATTGTC
AGGCAGGAGTATGGCATGAAACTCCCGGATGCGATCATTCTGGCCACTGCACAAGTCCATCGTTGTGAGCTTGTGACGCG
CAATACAAAAGACTTTGCCGATATTCCGGGAGTGATCACGCCGTATCACTTGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9181.38 Da
• Isoelectric Point: 5.6864

>AT224657 WP_036743750.1 NZ_CP087021:13247-13498 [Pseudosulfitobacter pseudonitzschiae]
MDVMTYSDARAQLKGVMDRAIHDKQEVVVTRKKGESVVVVSLDTWNAVNETLHLLSTPKNASRLRASIAQLDAGTGEERE
LTE

Nucleotide

• Length: 252 bp

>AT224657 NZ_CP120392:c4678862-4678620 [Salmonella enterica subsp. enterica serovar Typhi]
ATGATGGCTGGGATGGATATGGGCAGAATTTTACTCGATTTATCAGACGATGTGATTAAACGGCTTGACGATCTCAAGGT
GCAACGCAACCTACCTCGTGCAGAACTCTTGCGGGAGGCCGTTGAGCAGTATCTGGAAAGACAGGATCGGGCTGAGACCA
CAATTTCGAAAGCATTGGGGCTCTGGCAAGGCTGTGAGGAAGATGGCGTTGAATACCAACGTAAACTTCGTGAGGAATGG
TAA

Structures

Antitoxin

• AlphaFold DB: A0A074T9H5